Skip to Main Content
Table 1.

Summary of the terms used to describe the experimental protocol

TermExplanation
Experimental Treatment All birds (N=20) were initially assigned either as Flyers or Controls. One assigned Flyer was excluded due to illness, leaving a total of 19 birds. 
    Flyers Birds assigned to fly in the wind tunnel and successfully flew the equivalent of about 1500 km during 1 week (N=6). 
    Controls Birds assigned not to fly in the wind tunnel (N=8). 
    Failed Flyers Birds originally assigned to be Flyers, but did not successfully complete the Flight Treatment (N=5). 
Flight Training 1 week of training in the wind tunnel of the birds assigned as Flyers. 
Flight Treatment The 1 week of long flights in the wind tunnel following the Secondary injection. 
Batch Batch 1 and 2 (N=10 and N=9) started their Experimental Treatment 11 and 57 days after capture, respectively. 
Immune Challenges First and second injections of diphtheria—tetanus (DT) antigens in the breast muscle; phytohaemaggluttinin (PHA) injection in the wing web. 
Cell-mediated response Measured as skin-swelling response 24 h after injection of PHA in the wing web. 
Humoral responses Measured as antigen-specific antibody levels in blood before or a number of days after a DT injection in breast muscle. 
    Baseline response Antibody levels measured from blood sample taken just before the first DT injection. 
    Primary response Antibody levels measured from blood sample taken 14 days after the first DT injection. 
    Secondary response Antibody levels measured from blood sample taken 8 days after the second DT injection. 
Corticosterone levels Corticosterone levels in blood sampled at certain occasions during the experimental protocol. 
    PreTreat Corticosterone levels 1 day after the birds arrived to the wind tunnel. 
    PostTreat Corticosterone levels the day after the Flight Treatment ended. Sampled at the same time as the Secondary response. 
    Baseline Corticosterone levels within 3 min from catching a bird. 
    Stress-induced Corticosterone levels ca. 30 min from catching a bird. 
TermExplanation
Experimental Treatment All birds (N=20) were initially assigned either as Flyers or Controls. One assigned Flyer was excluded due to illness, leaving a total of 19 birds. 
    Flyers Birds assigned to fly in the wind tunnel and successfully flew the equivalent of about 1500 km during 1 week (N=6). 
    Controls Birds assigned not to fly in the wind tunnel (N=8). 
    Failed Flyers Birds originally assigned to be Flyers, but did not successfully complete the Flight Treatment (N=5). 
Flight Training 1 week of training in the wind tunnel of the birds assigned as Flyers. 
Flight Treatment The 1 week of long flights in the wind tunnel following the Secondary injection. 
Batch Batch 1 and 2 (N=10 and N=9) started their Experimental Treatment 11 and 57 days after capture, respectively. 
Immune Challenges First and second injections of diphtheria—tetanus (DT) antigens in the breast muscle; phytohaemaggluttinin (PHA) injection in the wing web. 
Cell-mediated response Measured as skin-swelling response 24 h after injection of PHA in the wing web. 
Humoral responses Measured as antigen-specific antibody levels in blood before or a number of days after a DT injection in breast muscle. 
    Baseline response Antibody levels measured from blood sample taken just before the first DT injection. 
    Primary response Antibody levels measured from blood sample taken 14 days after the first DT injection. 
    Secondary response Antibody levels measured from blood sample taken 8 days after the second DT injection. 
Corticosterone levels Corticosterone levels in blood sampled at certain occasions during the experimental protocol. 
    PreTreat Corticosterone levels 1 day after the birds arrived to the wind tunnel. 
    PostTreat Corticosterone levels the day after the Flight Treatment ended. Sampled at the same time as the Secondary response. 
    Baseline Corticosterone levels within 3 min from catching a bird. 
    Stress-induced Corticosterone levels ca. 30 min from catching a bird. 
Close Modal

or Create an Account

Close Modal
Close Modal