Term . | Explanation . |
---|---|
Experimental Treatment | All birds (N=20) were initially assigned either as Flyers or Controls. One assigned Flyer was excluded due to illness, leaving a total of 19 birds. |
Flyers | Birds assigned to fly in the wind tunnel and successfully flew the equivalent of about 1500 km during 1 week (N=6). |
Controls | Birds assigned not to fly in the wind tunnel (N=8). |
Failed Flyers | Birds originally assigned to be Flyers, but did not successfully complete the Flight Treatment (N=5). |
Flight Training | 1 week of training in the wind tunnel of the birds assigned as Flyers. |
Flight Treatment | The 1 week of long flights in the wind tunnel following the Secondary injection. |
Batch | Batch 1 and 2 (N=10 and N=9) started their Experimental Treatment 11 and 57 days after capture, respectively. |
Immune Challenges | First and second injections of diphtheria—tetanus (DT) antigens in the breast muscle; phytohaemaggluttinin (PHA) injection in the wing web. |
Cell-mediated response | Measured as skin-swelling response 24 h after injection of PHA in the wing web. |
Humoral responses | Measured as antigen-specific antibody levels in blood before or a number of days after a DT injection in breast muscle. |
Baseline response | Antibody levels measured from blood sample taken just before the first DT injection. |
Primary response | Antibody levels measured from blood sample taken 14 days after the first DT injection. |
Secondary response | Antibody levels measured from blood sample taken 8 days after the second DT injection. |
Corticosterone levels | Corticosterone levels in blood sampled at certain occasions during the experimental protocol. |
PreTreat | Corticosterone levels 1 day after the birds arrived to the wind tunnel. |
PostTreat | Corticosterone levels the day after the Flight Treatment ended. Sampled at the same time as the Secondary response. |
Baseline | Corticosterone levels within 3 min from catching a bird. |
Stress-induced | Corticosterone levels ca. 30 min from catching a bird. |
Term . | Explanation . |
---|---|
Experimental Treatment | All birds (N=20) were initially assigned either as Flyers or Controls. One assigned Flyer was excluded due to illness, leaving a total of 19 birds. |
Flyers | Birds assigned to fly in the wind tunnel and successfully flew the equivalent of about 1500 km during 1 week (N=6). |
Controls | Birds assigned not to fly in the wind tunnel (N=8). |
Failed Flyers | Birds originally assigned to be Flyers, but did not successfully complete the Flight Treatment (N=5). |
Flight Training | 1 week of training in the wind tunnel of the birds assigned as Flyers. |
Flight Treatment | The 1 week of long flights in the wind tunnel following the Secondary injection. |
Batch | Batch 1 and 2 (N=10 and N=9) started their Experimental Treatment 11 and 57 days after capture, respectively. |
Immune Challenges | First and second injections of diphtheria—tetanus (DT) antigens in the breast muscle; phytohaemaggluttinin (PHA) injection in the wing web. |
Cell-mediated response | Measured as skin-swelling response 24 h after injection of PHA in the wing web. |
Humoral responses | Measured as antigen-specific antibody levels in blood before or a number of days after a DT injection in breast muscle. |
Baseline response | Antibody levels measured from blood sample taken just before the first DT injection. |
Primary response | Antibody levels measured from blood sample taken 14 days after the first DT injection. |
Secondary response | Antibody levels measured from blood sample taken 8 days after the second DT injection. |
Corticosterone levels | Corticosterone levels in blood sampled at certain occasions during the experimental protocol. |
PreTreat | Corticosterone levels 1 day after the birds arrived to the wind tunnel. |
PostTreat | Corticosterone levels the day after the Flight Treatment ended. Sampled at the same time as the Secondary response. |
Baseline | Corticosterone levels within 3 min from catching a bird. |
Stress-induced | Corticosterone levels ca. 30 min from catching a bird. |