Group 1 | TM10 transplanted at 3 weeks of age. No DMBA. |
Group 2 | TM10 transplanted at 3 weeks of age and DMBA given at 8 weeks of host age. |
Group 3 | TM10 transplanted at 11 weeks of host age into untreated cleared fat pads. |
Group 4 | TM10 transplanted at 11 weeks of age into DMBA-treated cleared fat pads. |
Group 5 | TM10 transplants that had been treated with DMBA (group 2) transplanted at 11 weeks of age into untreated cleared fat pads. |
Group 6 | TM10 transplants that had been treated with DMBA (group 2) transplanted at 11 weeks into DMBA-treated cleared fat pads. |
Group 7 | TM10 transplanted at 11 weeks of age (untreated fat pad — group 3), then transplanted as a whole gland into an age-matched untreated host at 12 weeks. |
Group 8 | TM10 transplanted at 11 weeks (DMBA-treated fat pad — group 4), then transplanted as a whole gland into an age-matched untreated host at 12 weeks. |
Group 1 | TM10 transplanted at 3 weeks of age. No DMBA. |
Group 2 | TM10 transplanted at 3 weeks of age and DMBA given at 8 weeks of host age. |
Group 3 | TM10 transplanted at 11 weeks of host age into untreated cleared fat pads. |
Group 4 | TM10 transplanted at 11 weeks of age into DMBA-treated cleared fat pads. |
Group 5 | TM10 transplants that had been treated with DMBA (group 2) transplanted at 11 weeks of age into untreated cleared fat pads. |
Group 6 | TM10 transplants that had been treated with DMBA (group 2) transplanted at 11 weeks into DMBA-treated cleared fat pads. |
Group 7 | TM10 transplanted at 11 weeks of age (untreated fat pad — group 3), then transplanted as a whole gland into an age-matched untreated host at 12 weeks. |
Group 8 | TM10 transplanted at 11 weeks (DMBA-treated fat pad — group 4), then transplanted as a whole gland into an age-matched untreated host at 12 weeks. |
All mice had both inguinal fat pads cleared of epithelial rudiments at 3 weeks of age. Donor transplants were from TM10 hyperplastic mammary outgrowth line. Mice were palpated weekly for tumors for 52 weeks post transplantation.