Table 1.

Primers used for RT-PCR

Gene GenBank accession no. Primer sequences (upstream/downstream) Optimal annealing temperature Amplicon size (bp) Amplified segment (codons) Diagnostic restriction enzyme Fragment sizes (bp)
Aprt  M11310   5′-GTCTTCCCCGACTTCCCAATC-3′   61°C   317   15-119  XbaI   171, 146  
   5′-CCGGGTTCCAAGGCATCTTT-3′       
Kit  Y00864   5′-ACTCGCACGGGCACATACA-3′   60°C   356   91-216  DdeI   60, 296  
   5′-ATGGCTTCCCGCACTTTGAG-3′       
Kitl  S40364, S40534   5′-ATCGCGGGAATCCTGT-3′   53°C   396   28-167  HaeIII   92, 304  
   5′-GTCAGATGCCACCATAAAGTC-3′       
Gdf9  L06444   5′-TCCTTCAACCTCAGCGAATA-3′   56°C   369   323-445  HincII   282, 87  
   5′-GCCCCCATGCTAACGAC-3′       
Gene GenBank accession no. Primer sequences (upstream/downstream) Optimal annealing temperature Amplicon size (bp) Amplified segment (codons) Diagnostic restriction enzyme Fragment sizes (bp)
Aprt  M11310   5′-GTCTTCCCCGACTTCCCAATC-3′   61°C   317   15-119  XbaI   171, 146  
   5′-CCGGGTTCCAAGGCATCTTT-3′       
Kit  Y00864   5′-ACTCGCACGGGCACATACA-3′   60°C   356   91-216  DdeI   60, 296  
   5′-ATGGCTTCCCGCACTTTGAG-3′       
Kitl  S40364, S40534   5′-ATCGCGGGAATCCTGT-3′   53°C   396   28-167  HaeIII   92, 304  
   5′-GTCAGATGCCACCATAAAGTC-3′       
Gdf9  L06444   5′-TCCTTCAACCTCAGCGAATA-3′   56°C   369   323-445  HincII   282, 87  
   5′-GCCCCCATGCTAACGAC-3′       
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