PCR primer sequences for amplification of possum NBCs
| Primer name . | Primer sequence (5′–3′) . |
|---|---|
| Primers for cloning and expression | |
| NBC-For 1 | ATCAGTGATTTTGCCATTAT |
| NBC-Rev 1 | ATGTGAGCAATGGAGATGAC |
| NBC-For 2 | GCCTTGCTGGTCACCATCCTGATT |
| NBC-Rev 2 | TGGGCTACTTGCACTGGAGA |
| pNBC-For 1 | CAARCTGGAGGAGCGACGGAAG |
| kNBC-For 1 | ATCCCACCWRTSTTTAACC |
| kNBC-Rev 1 | CCGAGGTAAAAATACTACGGTTAAAGA |
| pNBC-For 2 | ATGGAGGATGAAGCCGTCCTG |
| kNBC-For 2 | ACCAAATTGAAGCTGGGATCT |
| Primers for in situ probe | |
| Forward | TGGAGGATGAAGCTGTCCTG |
| Reverse | TGAGTGGTTTGAGGATGCTAC |
| Primer name . | Primer sequence (5′–3′) . |
|---|---|
| Primers for cloning and expression | |
| NBC-For 1 | ATCAGTGATTTTGCCATTAT |
| NBC-Rev 1 | ATGTGAGCAATGGAGATGAC |
| NBC-For 2 | GCCTTGCTGGTCACCATCCTGATT |
| NBC-Rev 2 | TGGGCTACTTGCACTGGAGA |
| pNBC-For 1 | CAARCTGGAGGAGCGACGGAAG |
| kNBC-For 1 | ATCCCACCWRTSTTTAACC |
| kNBC-Rev 1 | CCGAGGTAAAAATACTACGGTTAAAGA |
| pNBC-For 2 | ATGGAGGATGAAGCCGTCCTG |
| kNBC-For 2 | ACCAAATTGAAGCTGGGATCT |
| Primers for in situ probe | |
| Forward | TGGAGGATGAAGCTGTCCTG |
| Reverse | TGAGTGGTTTGAGGATGCTAC |