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Table 1.

List of primers used for cloning, screening and real-time PCR for urea transporter (lfUT) and actin of Protopterus annectens

Primers5′–3′
Cloning primers  
   UT_F1 TgC AgT gTC AAC TCT TgT TgC 
   UT_R1 gTT gAA gTg TCC AgT TgC AgC 
   dgActin_F CAg ggY gTS ATg gTK ggB AT 
   dgActin_R KgT Tgg CYT Tgg gRT TSA g 
RACE primers  
   PA_UT_3′_GSP ggC CTC CAT ggA TAC AAT ggg ATC CTT gTg g 
   PA_UT_3′_NGSP CCC AgT ggT TgT CAT gTC TgC AgT CTg TCC 
   PA_UT_5′_GSP gTg ATg TTA ggT ACT gAT gAC AAT CgC TgA 
   PA_UT_5′_NGSP CAg TTg CAg CTA TAT gCA ggC ATA CTg CAg 
Tissue screening primers  
   PA_UT_SF TgC CAC TgC TAg TCA gAg C 
   PA_UT_SR AgC AgT gCT gAg Agg CAg A 
Real-time PCR primers  
   PA_UT_QF CTg gTg gCT gAT TCT CCC AgT gg 
   PA_UT_QR gTC CAg TTg CAg CTA TAT gCA ggC A 
   PA_Actin_QF AgC CCA gAg CAA gAg Agg TAT CC 
   PA_Actin_QR TTg ggg TTC AgT ggA gCC TCT g 
Primers5′–3′
Cloning primers  
   UT_F1 TgC AgT gTC AAC TCT TgT TgC 
   UT_R1 gTT gAA gTg TCC AgT TgC AgC 
   dgActin_F CAg ggY gTS ATg gTK ggB AT 
   dgActin_R KgT Tgg CYT Tgg gRT TSA g 
RACE primers  
   PA_UT_3′_GSP ggC CTC CAT ggA TAC AAT ggg ATC CTT gTg g 
   PA_UT_3′_NGSP CCC AgT ggT TgT CAT gTC TgC AgT CTg TCC 
   PA_UT_5′_GSP gTg ATg TTA ggT ACT gAT gAC AAT CgC TgA 
   PA_UT_5′_NGSP CAg TTg CAg CTA TAT gCA ggC ATA CTg CAg 
Tissue screening primers  
   PA_UT_SF TgC CAC TgC TAg TCA gAg C 
   PA_UT_SR AgC AgT gCT gAg Agg CAg A 
Real-time PCR primers  
   PA_UT_QF CTg gTg gCT gAT TCT CCC AgT gg 
   PA_UT_QR gTC CAg TTg CAg CTA TAT gCA ggC A 
   PA_Actin_QF AgC CCA gAg CAA gAg Agg TAT CC 
   PA_Actin_QR TTg ggg TTC AgT ggA gCC TCT g 
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