List of primers used for cloning, screening and real-time PCR for urea transporter (lfUT) and actin of Protopterus annectens
| Primers . | 5′–3′ . |
|---|---|
| Cloning primers | |
| UT_F1 | TgC AgT gTC AAC TCT TgT TgC |
| UT_R1 | gTT gAA gTg TCC AgT TgC AgC |
| dgActin_F | CAg ggY gTS ATg gTK ggB AT |
| dgActin_R | KgT Tgg CYT Tgg gRT TSA g |
| RACE primers | |
| PA_UT_3′_GSP | ggC CTC CAT ggA TAC AAT ggg ATC CTT gTg g |
| PA_UT_3′_NGSP | CCC AgT ggT TgT CAT gTC TgC AgT CTg TCC |
| PA_UT_5′_GSP | gTg ATg TTA ggT ACT gAT gAC AAT CgC TgA |
| PA_UT_5′_NGSP | CAg TTg CAg CTA TAT gCA ggC ATA CTg CAg |
| Tissue screening primers | |
| PA_UT_SF | TgC CAC TgC TAg TCA gAg C |
| PA_UT_SR | AgC AgT gCT gAg Agg CAg A |
| Real-time PCR primers | |
| PA_UT_QF | CTg gTg gCT gAT TCT CCC AgT gg |
| PA_UT_QR | gTC CAg TTg CAg CTA TAT gCA ggC A |
| PA_Actin_QF | AgC CCA gAg CAA gAg Agg TAT CC |
| PA_Actin_QR | TTg ggg TTC AgT ggA gCC TCT g |
| Primers . | 5′–3′ . |
|---|---|
| Cloning primers | |
| UT_F1 | TgC AgT gTC AAC TCT TgT TgC |
| UT_R1 | gTT gAA gTg TCC AgT TgC AgC |
| dgActin_F | CAg ggY gTS ATg gTK ggB AT |
| dgActin_R | KgT Tgg CYT Tgg gRT TSA g |
| RACE primers | |
| PA_UT_3′_GSP | ggC CTC CAT ggA TAC AAT ggg ATC CTT gTg g |
| PA_UT_3′_NGSP | CCC AgT ggT TgT CAT gTC TgC AgT CTg TCC |
| PA_UT_5′_GSP | gTg ATg TTA ggT ACT gAT gAC AAT CgC TgA |
| PA_UT_5′_NGSP | CAg TTg CAg CTA TAT gCA ggC ATA CTg CAg |
| Tissue screening primers | |
| PA_UT_SF | TgC CAC TgC TAg TCA gAg C |
| PA_UT_SR | AgC AgT gCT gAg Agg CAg A |
| Real-time PCR primers | |
| PA_UT_QF | CTg gTg gCT gAT TCT CCC AgT gg |
| PA_UT_QR | gTC CAg TTg CAg CTA TAT gCA ggC A |
| PA_Actin_QF | AgC CCA gAg CAA gAg Agg TAT CC |
| PA_Actin_QR | TTg ggg TTC AgT ggA gCC TCT g |