Characteristics of the intestinal antibodies
| Antibody name . | Antigen size on western blot (kDa) . | Expression first seen at (days) . | Structures labelled . |
|---|---|---|---|
| 2F11 | Four major bands at 170, 120, 90 and 35 | 3 | Cytoplasm of enteroendocrine and goblet cells (but no labelling of the mucus compartment). The 3 markers label identical cell types as determined by double labelling |
| 4B7/2 | 35 | 3 | |
| 6G5 | n.d. | 3 | |
| 3G12 | Three major bands at 175, 120 and 70 | 5 | Mucus compartment and cytoplasm of goblet cells, in the posterior gut mainly |
| 4B7/1 | n.d. | 3 | Brush border of enterocytes (apical ends of microvilli) |
| 4E8 | 200 | 3 | |
| 2H9 | 170 | 3 | Lateral membrane and basal region of epithelial cells |
| 5F11 | n.d. | 2 | Basement membrane |
| Antibody name . | Antigen size on western blot (kDa) . | Expression first seen at (days) . | Structures labelled . |
|---|---|---|---|
| 2F11 | Four major bands at 170, 120, 90 and 35 | 3 | Cytoplasm of enteroendocrine and goblet cells (but no labelling of the mucus compartment). The 3 markers label identical cell types as determined by double labelling |
| 4B7/2 | 35 | 3 | |
| 6G5 | n.d. | 3 | |
| 3G12 | Three major bands at 175, 120 and 70 | 5 | Mucus compartment and cytoplasm of goblet cells, in the posterior gut mainly |
| 4B7/1 | n.d. | 3 | Brush border of enterocytes (apical ends of microvilli) |
| 4E8 | 200 | 3 | |
| 2H9 | 170 | 3 | Lateral membrane and basal region of epithelial cells |
| 5F11 | n.d. | 2 | Basement membrane |
n.d., not determined.