Primers for RT-PCR and probe synthesis
| . | Primer . | Sequence (5′ to 3′) . | |
|---|---|---|---|
| Real-time RT-PCR primers | |||
| ERECTA | sbr ER F | GACTGGACAACTTCACCTTCTTCG | |
| sbr ER R | GAACAGTCACCAATCTCATCAGG | ||
| ERL1 | sbr ERL1 F | GAACTGCTTCACTCTTGTCAATCTG | |
| sbr ERL1 R | CATACCTGGCTGGAGCAAAACG | ||
| ERL2 | ERL2 3085 | CTGTCTGGCAACAATTTCTCA | |
| sbr ERL2 R | AACACGTACAGATTGGCAAGAC | ||
| ACTIN | sbr actin F | ATGTCGCCATCCAAGCTGTTCTC | |
| sbr actin r | CTCCTTGATGTCTCTTACAATTTCC | ||
| PFS2 | pfs2 F | ATGGGCTACATCTCCAACAA | |
| sbr PFS2 R | CTTCTAGCGTCGTGATCTGC | ||
| CYCLIN A2;2 | sbr CYCA22 F | GAGCTAAAAAAGCCATGGGAA | |
| sbr CYCA22 R | CACCTGATCCATCTTCTTGTTGC | ||
| CYCLIN A2;3 | CYCA23 21 | GTCTCGGCCATTCACTCG | |
| sbr CYCA23 R | TTTGGCCTCAAGTATACCTG | ||
| RT-PCR primers | |||
| CYCLIN A2;1 | CYCA21 153 | GAAGCGTGTTGCTAGACCG | |
| CYCA21 347.rc | CTACCAAACCATCTTCTTC | ||
| CYCLIN A2;2 | CYCA22 597 | GCTAAAAAAGCCATGGGAAGA | |
| CYCA22 1103.rc | TGCTCAGTAATTTCTTCATCTT | ||
| CYCLIN A2;3 | CYCA23 21 | GTCTCGGCCATTCACTCG | |
| CYCA23 359.RC | CCTGCTGTATTTGATGCAAC | ||
| CYCLIN A2;4 | CYCA24 (−26) | TTGGATCGTGTCAAAGGG | |
| CYCA24 518.rc | ACTTCTCTCAACTGTCCC | ||
| CDKB1;1 | cdkb11 610 | CAATGGCCGGGTGTTTCC | |
| cdkb11 +69.rc | TGTCGGAAAGAAAGATAG | ||
| CDKB1;2 | cdkb12 615 | CAATGGCCGGGTGTAATG | |
| cdkb12 +68.rc | GAGCAATAAACTTGACGG | ||
| CDKB2;1 | cdkb21 (−40) | AGAGAGAGAGAGCGCCG | |
| cdkb21 352.rc | GAATGTTCTTGCCAGTGCT | ||
| CDKB2;2 | cdkb22 (−32) | GAGAGAAGAGTTGTCTTG | |
| cdkb22 358.rc | GAATGTTCTGTCCAGCTTG | ||
| ERECTA | ER 2248 | AAGAAGTCATTCAAAGATGTGA | |
| ER3016.rc | AGAATTTCCAGGTTTGGAATCTGT | ||
| ERL1 | ERL1 2846 | TATCCCACCGATACTTGGCA | |
| ERL1 4411.rc | CCGGAGAGATTGTTGAAGGA | ||
| ERL2 | ERL2 3085 | CTGTCTGGCAACAATTTCTCA | |
| ERL2 4254.rc | AGCCATGTCCATGTGAAGAA | ||
| ACTIN 2 | act2-1 | GCCATCCAAGCTGTTCTCTC | |
| act2-2 | GCTCGTAGTCAACAGCAACAA | ||
| PFS2 | pfs2 F | ATGGGCTACATCTCCAACAA | |
| pfs2 R | TCAGTTCTTCAGAGGCATGA | ||
| In situ hybridization probe primers | |||
| ERECTA | ER 7040 | GTAAAGATCTCGGTGTGG | |
| ER 7467.rc | GAAGACACATATTCACACC | ||
| . | Primer . | Sequence (5′ to 3′) . | |
|---|---|---|---|
| Real-time RT-PCR primers | |||
| ERECTA | sbr ER F | GACTGGACAACTTCACCTTCTTCG | |
| sbr ER R | GAACAGTCACCAATCTCATCAGG | ||
| ERL1 | sbr ERL1 F | GAACTGCTTCACTCTTGTCAATCTG | |
| sbr ERL1 R | CATACCTGGCTGGAGCAAAACG | ||
| ERL2 | ERL2 3085 | CTGTCTGGCAACAATTTCTCA | |
| sbr ERL2 R | AACACGTACAGATTGGCAAGAC | ||
| ACTIN | sbr actin F | ATGTCGCCATCCAAGCTGTTCTC | |
| sbr actin r | CTCCTTGATGTCTCTTACAATTTCC | ||
| PFS2 | pfs2 F | ATGGGCTACATCTCCAACAA | |
| sbr PFS2 R | CTTCTAGCGTCGTGATCTGC | ||
| CYCLIN A2;2 | sbr CYCA22 F | GAGCTAAAAAAGCCATGGGAA | |
| sbr CYCA22 R | CACCTGATCCATCTTCTTGTTGC | ||
| CYCLIN A2;3 | CYCA23 21 | GTCTCGGCCATTCACTCG | |
| sbr CYCA23 R | TTTGGCCTCAAGTATACCTG | ||
| RT-PCR primers | |||
| CYCLIN A2;1 | CYCA21 153 | GAAGCGTGTTGCTAGACCG | |
| CYCA21 347.rc | CTACCAAACCATCTTCTTC | ||
| CYCLIN A2;2 | CYCA22 597 | GCTAAAAAAGCCATGGGAAGA | |
| CYCA22 1103.rc | TGCTCAGTAATTTCTTCATCTT | ||
| CYCLIN A2;3 | CYCA23 21 | GTCTCGGCCATTCACTCG | |
| CYCA23 359.RC | CCTGCTGTATTTGATGCAAC | ||
| CYCLIN A2;4 | CYCA24 (−26) | TTGGATCGTGTCAAAGGG | |
| CYCA24 518.rc | ACTTCTCTCAACTGTCCC | ||
| CDKB1;1 | cdkb11 610 | CAATGGCCGGGTGTTTCC | |
| cdkb11 +69.rc | TGTCGGAAAGAAAGATAG | ||
| CDKB1;2 | cdkb12 615 | CAATGGCCGGGTGTAATG | |
| cdkb12 +68.rc | GAGCAATAAACTTGACGG | ||
| CDKB2;1 | cdkb21 (−40) | AGAGAGAGAGAGCGCCG | |
| cdkb21 352.rc | GAATGTTCTTGCCAGTGCT | ||
| CDKB2;2 | cdkb22 (−32) | GAGAGAAGAGTTGTCTTG | |
| cdkb22 358.rc | GAATGTTCTGTCCAGCTTG | ||
| ERECTA | ER 2248 | AAGAAGTCATTCAAAGATGTGA | |
| ER3016.rc | AGAATTTCCAGGTTTGGAATCTGT | ||
| ERL1 | ERL1 2846 | TATCCCACCGATACTTGGCA | |
| ERL1 4411.rc | CCGGAGAGATTGTTGAAGGA | ||
| ERL2 | ERL2 3085 | CTGTCTGGCAACAATTTCTCA | |
| ERL2 4254.rc | AGCCATGTCCATGTGAAGAA | ||
| ACTIN 2 | act2-1 | GCCATCCAAGCTGTTCTCTC | |
| act2-2 | GCTCGTAGTCAACAGCAACAA | ||
| PFS2 | pfs2 F | ATGGGCTACATCTCCAACAA | |
| pfs2 R | TCAGTTCTTCAGAGGCATGA | ||
| In situ hybridization probe primers | |||
| ERECTA | ER 7040 | GTAAAGATCTCGGTGTGG | |
| ER 7467.rc | GAAGACACATATTCACACC | ||