Phenotype of Ppa-pax-3, Ppa-lin-39 and double mutants in the hermaphrodite ventral epidermis
| Genotype . | P(1-4).p . | P5.p . | P6.p . | P7.p . | P8.p . | P9.p . | P10.p . | P11.p . | SI . | n . |
|---|---|---|---|---|---|---|---|---|---|---|
| A. Wild type PS312 | 0:0 | 100:100 | 100:100 | 100:100 | 100:0 | 0:0 | 0:0 | 0:0 | 4 | many |
| B. Ppa-lin-39(tu29) | 0:0 | 13:13 | 44:38 | 19:13 | 19:0 | 0:0 | 0:0 | 0:0 | 0.9 | 16 |
| C. Ppa-pax-3(tu214) | 0:0 | 34:12 | 48:31 | 48:17 | 57:0 | 16:0 | 14:0 | 22:7 | 1.9 | 50 |
| D. Ppa-pax-3(tu358) | 0:0 | 43:43 | 52:48 | 57:55 | 62:0 | 10:0 | 7:0 | 17:6 | 2.2 | 42 |
| E. Ppa-lin-39(tu29)/Ppa-ced-3(tu84) | 100:0 | 100:100 | 100:100 | 100:100 | 100:0 | 100:0 | 100:0 | 100:100* | 11 | 15 |
| F. Ppa-pax-3(tu358)/Ppa-ced-4(tu324) | 100:0 | 100:100 | 100:100 | 100:100 | 100:0 | 100:0 | 100:0 | 100:90* | 11 | 19 |
| G. Ppa-pax-3(tu214)/Ppa-ced-4(tu324) | 100:0 | 100:80 | 100:80 | 100:73 | 100:0 | 100:0 | 100:0 | 100:90* | 11 | 15 |
| H. Ppa-pax-3(tu214)/Ppa-pax-3(tu358) | 0:0 | 32:21 | 47:32 | 47:32 | 53:0 | 11:0 | 11:0 | 21:5 | 1.8 | 19 |
| I. Ppa-pax-3 MO | 0:0 | 100:100 | 100:100 | 100:100 | 100:0 | 0:0 | 0:0 | 0:0 | 4 | 50 |
| J. Ppa-pax-3(tu358)/Ppa-pax-3 MO | 0:0 | 17:7 | 23:20 | 23:10 | 23:0 | 33:0 | 23:0 | 43:27 | 0.9 | 30 |
| K. Ppa-pax-3(tu214)/Ppa-lin-39(tu29) | 0:0 | 25:7 | 32:18 | 18:4 | 11:0 | 0:0 | 0:0 | 0:0 | 0.9 | 28 |
| Genotype . | P(1-4).p . | P5.p . | P6.p . | P7.p . | P8.p . | P9.p . | P10.p . | P11.p . | SI . | n . |
|---|---|---|---|---|---|---|---|---|---|---|
| A. Wild type PS312 | 0:0 | 100:100 | 100:100 | 100:100 | 100:0 | 0:0 | 0:0 | 0:0 | 4 | many |
| B. Ppa-lin-39(tu29) | 0:0 | 13:13 | 44:38 | 19:13 | 19:0 | 0:0 | 0:0 | 0:0 | 0.9 | 16 |
| C. Ppa-pax-3(tu214) | 0:0 | 34:12 | 48:31 | 48:17 | 57:0 | 16:0 | 14:0 | 22:7 | 1.9 | 50 |
| D. Ppa-pax-3(tu358) | 0:0 | 43:43 | 52:48 | 57:55 | 62:0 | 10:0 | 7:0 | 17:6 | 2.2 | 42 |
| E. Ppa-lin-39(tu29)/Ppa-ced-3(tu84) | 100:0 | 100:100 | 100:100 | 100:100 | 100:0 | 100:0 | 100:0 | 100:100* | 11 | 15 |
| F. Ppa-pax-3(tu358)/Ppa-ced-4(tu324) | 100:0 | 100:100 | 100:100 | 100:100 | 100:0 | 100:0 | 100:0 | 100:90* | 11 | 19 |
| G. Ppa-pax-3(tu214)/Ppa-ced-4(tu324) | 100:0 | 100:80 | 100:80 | 100:73 | 100:0 | 100:0 | 100:0 | 100:90* | 11 | 15 |
| H. Ppa-pax-3(tu214)/Ppa-pax-3(tu358) | 0:0 | 32:21 | 47:32 | 47:32 | 53:0 | 11:0 | 11:0 | 21:5 | 1.8 | 19 |
| I. Ppa-pax-3 MO | 0:0 | 100:100 | 100:100 | 100:100 | 100:0 | 0:0 | 0:0 | 0:0 | 4 | 50 |
| J. Ppa-pax-3(tu358)/Ppa-pax-3 MO | 0:0 | 17:7 | 23:20 | 23:10 | 23:0 | 33:0 | 23:0 | 43:27 | 0.9 | 30 |
| K. Ppa-pax-3(tu214)/Ppa-lin-39(tu29) | 0:0 | 25:7 | 32:18 | 18:4 | 11:0 | 0:0 | 0:0 | 0:0 | 0.9 | 28 |
The ratios for the P5.p to P11.p cells give the percentage of surviving cells to the percentage of the cells differentiating into vulval (1° or 2°) tissue. *Note that, in the cell death defective genetic background of Ppa-ced-3 and Ppa-ced-4, P11.p undergoes differentiation and forms an invagination in the pre-anal region (see Sommer et al., 1998). SI,survival index describes the number of surviving P(5-8).p cells per animal; n, number of analyzed animals.