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Table 1.

Primers for RT-PCR

Gene	Forward primer 5′-3′	Reverse primer 5′-3′	Cycles	References
Axin	AAGGCCAGACCTAGACTTGG	CGAACTGTCTAACGCAAGC	32	Hedgepeth et al., 1999
Bra	GCTGGAAGTATGTGAATGGAG	TTAAGTGCTGTAATCTCTTCA	32	Agius et al., 2000
CD44	CAAGCGCGTACAGTTATCAGC	TACAGAGTCTCCCTGGTATCC	32	Ori et al., 2006
Coll2	AGGCTTGGCTGGTCCTCAAGGT	TGTAACGCATAGGGTCGGGTCC	30	Sasai et al., 1996
Delta-1	AAGCCCAGGTACCCTTCTGT	GGCAGAGTCTGGTCGTCTTC	30	Abe et al., 2004
EF1α	CCTGAACCACCCAGGCCAGATTGGTG	GAGGGTAGTCAGAGAAGCTCTCCACG	20-22	Agius et al., 2000
FGF8	CTGCGTCTTCTCGGAAATTGTC	GTTTTTATGAAGTCTGTGGAACG	32	Lin and Slack, 2008
Has1	CATGAAGCCATTCTCTTCTC	ACACGTCTTTGAACATCTCC	30	Unpublished
Has2	GTAACGCAGATGGTTCTCTC	GTTAAACGCCATCCAGTATC	28-30	Unpublished
Has3	GGGACAAAAATTACCATGAA	CAGAAATGAGATCCACGATT	35	Unpublished
Hyal2	TGCAGAGGACATTGCTGTTC	TAAGAGGTGATGGGGTCAGC	32	Vigetti et al., 2003
Msx1	GCTAAAAATGGCTGCTAA	AGGTGGGCTGTGTAAAGT	30	Unpublished^{^*}
Msx2	CACCTTCATTTAGGGATCAG	GTTTTGTGCTTCCTCAAAGT	30	Unpublished
RHAMM	CAAACAAGTGGCGCACCC	AGTCATTGCCCAGTCAGC	32	Groen et al., 2004
Shh	CTTCGCTCGGACGAGATGCTGG	CCTTCGTATCTGCCGCTGGCC	30	Sigiura et al., 2004
Syn-1	TACATCTGCGTTGCCTTCTG	TGGATTTCACAGTGGTTGGA	30	Olivares et al., 2009
Wnt5a	AAGGACTCTCCCATCTTGTT	AGAAAAGGCACTTCACACAG	30	Sigiura et al., 2004

Gene	Forward primer 5′-3′	Reverse primer 5′-3′	Cycles	References
Axin	AAGGCCAGACCTAGACTTGG	CGAACTGTCTAACGCAAGC	32	Hedgepeth et al., 1999
Bra	GCTGGAAGTATGTGAATGGAG	TTAAGTGCTGTAATCTCTTCA	32	Agius et al., 2000
CD44	CAAGCGCGTACAGTTATCAGC	TACAGAGTCTCCCTGGTATCC	32	Ori et al., 2006
Coll2	AGGCTTGGCTGGTCCTCAAGGT	TGTAACGCATAGGGTCGGGTCC	30	Sasai et al., 1996
Delta-1	AAGCCCAGGTACCCTTCTGT	GGCAGAGTCTGGTCGTCTTC	30	Abe et al., 2004
EF1α	CCTGAACCACCCAGGCCAGATTGGTG	GAGGGTAGTCAGAGAAGCTCTCCACG	20-22	Agius et al., 2000
FGF8	CTGCGTCTTCTCGGAAATTGTC	GTTTTTATGAAGTCTGTGGAACG	32	Lin and Slack, 2008
Has1	CATGAAGCCATTCTCTTCTC	ACACGTCTTTGAACATCTCC	30	Unpublished
Has2	GTAACGCAGATGGTTCTCTC	GTTAAACGCCATCCAGTATC	28-30	Unpublished
Has3	GGGACAAAAATTACCATGAA	CAGAAATGAGATCCACGATT	35	Unpublished
Hyal2	TGCAGAGGACATTGCTGTTC	TAAGAGGTGATGGGGTCAGC	32	Vigetti et al., 2003
Msx1	GCTAAAAATGGCTGCTAA	AGGTGGGCTGTGTAAAGT	30	Unpublished^{^*}
Msx2	CACCTTCATTTAGGGATCAG	GTTTTGTGCTTCCTCAAAGT	30	Unpublished
RHAMM	CAAACAAGTGGCGCACCC	AGTCATTGCCCAGTCAGC	32	Groen et al., 2004
Shh	CTTCGCTCGGACGAGATGCTGG	CCTTCGTATCTGCCGCTGGCC	30	Sigiura et al., 2004
Syn-1	TACATCTGCGTTGCCTTCTG	TGGATTTCACAGTGGTTGGA	30	Olivares et al., 2009
Wnt5a	AAGGACTCTCCCATCTTGTT	AGAAAAGGCACTTCACACAG	30	Sigiura et al., 2004