Primer characteristics used to amplify various sequences ofbeta-actin and hsp70 genes
| Primer . | Sequence . |
|---|---|
| (a) Conserved hsp70 | for 5′-AT(C/T) AA(C/T) GA(G/A) CCI AC(G/T) GCI GCI GC(T/A) ATT GCI TAT GG-3′ rev 5′-GA(T/C) GAA (A/G)G C(T/A/C)G TTG C(A/G)T A(T/C)G G(A/T/C)G C(A/T)G C(T/A/C)G TAC AAG C-3′ |
| (b) Conserved beta-actin | for 5′-CAT TGC CGA CAG GAT CCA GA-3′ rev 5′-GAC TCG TCG TAC TCC TGC TTG-3′ |
| (c) hsp70 isoform 1 | for 5′-CTT ATC AGT GAA ATT AAG CGA GAG C-3′ rev 5′-ACA AGG ATA ACT TCA TCA ACC TTT G-3′ |
| (d) hsp70 isoform 2 | for 5′-GAG GTG GTA CTT TTG ATG TTT CTG T-3′ rev 5′-GTC AAT TCC TTC GTA GAG AGA GTC A-3′ |
| (e) hsp70 isoform 3 | for 5′-TGG TGT TGA TAT TTC TAA AGA CCG T-3′ rev 5′-ACA AGG ATA ACT TCA TCA ACC TTT G-3′ |
| (f) beta-actin | for 5′-ATG AAG ATC AAG ATC ATC GCT CC-3′ rev 5′-GAG ATC CAC ATC TGC TGG AAG-3′ |
| Primer . | Sequence . |
|---|---|
| (a) Conserved hsp70 | for 5′-AT(C/T) AA(C/T) GA(G/A) CCI AC(G/T) GCI GCI GC(T/A) ATT GCI TAT GG-3′ rev 5′-GA(T/C) GAA (A/G)G C(T/A/C)G TTG C(A/G)T A(T/C)G G(A/T/C)G C(A/T)G C(T/A/C)G TAC AAG C-3′ |
| (b) Conserved beta-actin | for 5′-CAT TGC CGA CAG GAT CCA GA-3′ rev 5′-GAC TCG TCG TAC TCC TGC TTG-3′ |
| (c) hsp70 isoform 1 | for 5′-CTT ATC AGT GAA ATT AAG CGA GAG C-3′ rev 5′-ACA AGG ATA ACT TCA TCA ACC TTT G-3′ |
| (d) hsp70 isoform 2 | for 5′-GAG GTG GTA CTT TTG ATG TTT CTG T-3′ rev 5′-GTC AAT TCC TTC GTA GAG AGA GTC A-3′ |
| (e) hsp70 isoform 3 | for 5′-TGG TGT TGA TAT TTC TAA AGA CCG T-3′ rev 5′-ACA AGG ATA ACT TCA TCA ACC TTT G-3′ |
| (f) beta-actin | for 5′-ATG AAG ATC AAG ATC ATC GCT CC-3′ rev 5′-GAG ATC CAC ATC TGC TGG AAG-3′ |
for, forward; rev, reverse.