DNA transfected in HEK-293 cells . | EC50 (nmol l-1) . | Maximal response (%) . | r2 . |
---|---|---|---|
CG17415 + dRCP | 5219 | 189 | 0.9974 |
dRCP | NR | NR | NR |
CG17415 + hRCP | 116 | 1226 | 0.9857 |
hRCP | NR | NR | NR |
CG17415 + hRAMP1 | 108 | 936 | 0.9936 |
hRAMP1 | NR | NR | NR |
CG17415 + hRAMP2 | 101 | 897 | 0.9969 |
hRAMP2 | NR | NR | NR |
CG17415 + hRCP + hRAMP1 | 117 | 620 | 0.9991 |
hRCP + hRAMP1 | NR | NR | NR |
CG17415 + hRCP + hRAMP2 | 89 | 706 | 0.9941 |
hRCP + hRAMP2 | NR | NR | NR |
CG17415 + hRCP + hRAMP1 + hRAMP2 | 82 | 756 | 0.9981 |
hRCP + hRAMP1 + hRAMP2 | NR | NR | NR |
CG17415 + dRCP + hRAMP1 + hRAMP2 | 457 | 536 | 0.9894 |
dRCP + hRAMP1 + hRAMP2 | NR | NR | NR |
DNA transfected in HEK-293 cells . | EC50 (nmol l-1) . | Maximal response (%) . | r2 . |
---|---|---|---|
CG17415 + dRCP | 5219 | 189 | 0.9974 |
dRCP | NR | NR | NR |
CG17415 + hRCP | 116 | 1226 | 0.9857 |
hRCP | NR | NR | NR |
CG17415 + hRAMP1 | 108 | 936 | 0.9936 |
hRAMP1 | NR | NR | NR |
CG17415 + hRAMP2 | 101 | 897 | 0.9969 |
hRAMP2 | NR | NR | NR |
CG17415 + hRCP + hRAMP1 | 117 | 620 | 0.9991 |
hRCP + hRAMP1 | NR | NR | NR |
CG17415 + hRCP + hRAMP2 | 89 | 706 | 0.9941 |
hRCP + hRAMP2 | NR | NR | NR |
CG17415 + hRCP + hRAMP1 + hRAMP2 | 82 | 756 | 0.9981 |
hRCP + hRAMP1 + hRAMP2 | NR | NR | NR |
CG17415 + dRCP + hRAMP1 + hRAMP2 | 457 | 536 | 0.9894 |
dRCP + hRAMP1 + hRAMP2 | NR | NR | NR |
CG17415 cDNA was always transfected at a 5:1(GPCR:CRE-luc) ratio; any additional cDNAs were made equimolar with the CRE-luc reporter.
Data are pooled responses from three replicate wells from three independent transfections.
EC50 and r2 values were calculated from concentration–response curves using nonlinear curve fitting (PRISM 3.0,GraphPad, San Diego, CA).
NR, no statistically significant response (Excel t-test) at any dose.