Genotype . | Pharynx cells†(pha-4::GFP) . | Pharynx muscles‡(ceh-22::GFP) . | Muscle cells (hlh-1::GFP) . | Coelomocytes (cup-4::GFP) . |
---|---|---|---|---|
Wild type | 50.0±0.9 (21) | 12.8±0.1 (37) | 44.7±1.1 (20) | 3.7±0.2 (105) |
skn-1 (RNAi) | 4.8±0.4 (20) | 0.0±0.0 (165) | nd | 0.15±0.04 (124) |
pal-1 (RNAi) | 49.5±0.8 (10) | 11.7±0.3 (12) | 21.6±0.9 (13) | 3.7±0.1 (103) |
pop-1 (RNAi) | nd | nd | nd | 0.0±0.0 (50) |
glp-1 (RNAi) | 23.1±0.6 (15) | 5.7±0.2 (38) | nd | nd |
tbx-35(tm1789) 15°C | 40.6±1.2 (17) | 5.9±0.3 (24) | 37.3±1.6 (10) | 3.8±0.2 (28) |
tbx-35(tm1789) 23°C | 35.7±0.8 (16)** | 5.2±0.2 (46)* | 34.8±2.4 (10) | 3.3±0.4 (20) |
tbx-35(tm1789); glp-1 (RNAi) 15°C | 6.6±0.5 (23) | 2.0±0.4 (26) | nd | nd |
tbx-35(tm1789); glp-1 (RNAi) 23°C | 1.1±0.3 (32)** | 1.0±0.2 (39)* | nd | nd |
tbx-35(tm1789); pal-1 (RNAi) 15°C | 38.8±0.7 (15) | 5.1±0.3 (14) | 8.4±1.0 (17) | 2.2±0.2 (47) |
tbx-35(tm1789); pal-1 (RNAi) 23°C | 35.6±1.0 (14)* | 4.7±0.3 (17) | 5.7±0.5 (40)* | 0.6±0.1 (49)** |
ceh-51 (tm2123) | 47.8±0.9 (17) | 9.2±0.2 (10) | 42.4±1.4 (10) | 2.1±0.1 (53) |
ceh-51 (tm2123); pal-1 (RNAi) | nd | nd | 19.3±0.5 (11) | 2.5±0.1 (84) |
med-1 (ok804); med-2(cx9744) | 31.3±0.6 (26) | 4.1±0.2 (32) | 31.0±2.7 (10) | 0.07±0.03 (34) |
ceh-51 (tm2123); tbx-35(tm1789) | 30.2±0.5 (44) | 4.4±0.2 (18) | 30.1±1.0 (14) | 0.19±0.04 (124)* |
med-1 (ok804); med-2(cx9744); glp-1 (RNAi) | 1.4±0.4 (14) | 0.3±0.1 (31) | nd | nd |
ceh-51 (tm2123); tbx-35(tm1789); glp-1 (RNAi) | 1.9±0.5 (26) | 0.5±0.1 (52) | nd | nd |
med-1 (ok804); med-2(cx9744); pal-1 (RNAi) | nd | nd | 3.8±0.5 (13) | nd |
ceh-51 (tm2123); tbx-35(tm1789); pal-1 (RNAi) | nd | nd | 3.9±0.4 (15) | nd |
Genotype . | Pharynx cells†(pha-4::GFP) . | Pharynx muscles‡(ceh-22::GFP) . | Muscle cells (hlh-1::GFP) . | Coelomocytes (cup-4::GFP) . |
---|---|---|---|---|
Wild type | 50.0±0.9 (21) | 12.8±0.1 (37) | 44.7±1.1 (20) | 3.7±0.2 (105) |
skn-1 (RNAi) | 4.8±0.4 (20) | 0.0±0.0 (165) | nd | 0.15±0.04 (124) |
pal-1 (RNAi) | 49.5±0.8 (10) | 11.7±0.3 (12) | 21.6±0.9 (13) | 3.7±0.1 (103) |
pop-1 (RNAi) | nd | nd | nd | 0.0±0.0 (50) |
glp-1 (RNAi) | 23.1±0.6 (15) | 5.7±0.2 (38) | nd | nd |
tbx-35(tm1789) 15°C | 40.6±1.2 (17) | 5.9±0.3 (24) | 37.3±1.6 (10) | 3.8±0.2 (28) |
tbx-35(tm1789) 23°C | 35.7±0.8 (16)** | 5.2±0.2 (46)* | 34.8±2.4 (10) | 3.3±0.4 (20) |
tbx-35(tm1789); glp-1 (RNAi) 15°C | 6.6±0.5 (23) | 2.0±0.4 (26) | nd | nd |
tbx-35(tm1789); glp-1 (RNAi) 23°C | 1.1±0.3 (32)** | 1.0±0.2 (39)* | nd | nd |
tbx-35(tm1789); pal-1 (RNAi) 15°C | 38.8±0.7 (15) | 5.1±0.3 (14) | 8.4±1.0 (17) | 2.2±0.2 (47) |
tbx-35(tm1789); pal-1 (RNAi) 23°C | 35.6±1.0 (14)* | 4.7±0.3 (17) | 5.7±0.5 (40)* | 0.6±0.1 (49)** |
ceh-51 (tm2123) | 47.8±0.9 (17) | 9.2±0.2 (10) | 42.4±1.4 (10) | 2.1±0.1 (53) |
ceh-51 (tm2123); pal-1 (RNAi) | nd | nd | 19.3±0.5 (11) | 2.5±0.1 (84) |
med-1 (ok804); med-2(cx9744) | 31.3±0.6 (26) | 4.1±0.2 (32) | 31.0±2.7 (10) | 0.07±0.03 (34) |
ceh-51 (tm2123); tbx-35(tm1789) | 30.2±0.5 (44) | 4.4±0.2 (18) | 30.1±1.0 (14) | 0.19±0.04 (124)* |
med-1 (ok804); med-2(cx9744); glp-1 (RNAi) | 1.4±0.4 (14) | 0.3±0.1 (31) | nd | nd |
ceh-51 (tm2123); tbx-35(tm1789); glp-1 (RNAi) | 1.9±0.5 (26) | 0.5±0.1 (52) | nd | nd |
med-1 (ok804); med-2(cx9744); pal-1 (RNAi) | nd | nd | 3.8±0.5 (13) | nd |
ceh-51 (tm2123); tbx-35(tm1789); pal-1 (RNAi) | nd | nd | 3.9±0.4 (15) | nd |
Strains were grown at 20-23°C unless otherwise indicated. Data are shown as the mean ± s.e.m. *0.01<P<0.05, **P<0.01, Student's t-test, by comparison with the experiment immediately above. nd, not done.
Only pharynx expression of pha-4::GFP, anterior to the gut (when present), was scored.
The anatomy of the pharynx was considered in assigning expression to particular muscle cells.