βNHE activity and mean cellular haemoglobin concentration (MCHC)measured in density-separated red blood cells (RBCs) and in the original RBC suspension
| Cell fraction . | Na+ influx (mmol 1−1 RBCs h−1) . | MCHC (mmol 1−1) . |
|---|---|---|
| Top (young) | 125.6±9.5 | 3.82±0.16 |
| Middle (older) | 90.1±8.5** | 4.24±0.14* |
| Bottom (oldest) | 102.0±7.7* | 4.57±0.10*** |
| Original RBC suspension | 91.0±4.9** | 4.10±0.18* |
| Cell fraction . | Na+ influx (mmol 1−1 RBCs h−1) . | MCHC (mmol 1−1) . |
|---|---|---|
| Top (young) | 125.6±9.5 | 3.82±0.16 |
| Middle (older) | 90.1±8.5** | 4.24±0.14* |
| Bottom (oldest) | 102.0±7.7* | 4.57±0.10*** |
| Original RBC suspension | 91.0±4.9** | 4.10±0.18* |
Statistically significant differences from values for the top fraction are indicated by *P<0.05, **P<0.01 and ***P<0.001. N=6 for each experiment.