Hedgehog signalling affects HSC activity in AGM explants
| Embryonic day . | Tissue . | Factor or antibody . | Concentration (ng/ml) . | Number repopulated/number transplanted . | % Recipients repopulated . | % Chimerism . |
|---|---|---|---|---|---|---|
| Early E10 | AGM | Ihh | 2 | 0/9 | 0 | - |
| 20 | 4/10 | 40 | 100, 89, 99, 91 | |||
| 200 | 0/12 | 0 | - | |||
| Early E10 | AGM | Shh | 2 | 0/3 | 0 | - |
| 20 | 4/10 | 40 | 95, 89, 95, 33 | |||
| 200 | 0/8 | 0 | - | |||
| Early E10 | AGM:gut | IgG | 50 | 2/8 | 25 | 60, 99 |
| Anti-Hh | 50 | 0/7 | 0 | - | ||
| Late E10 | AGM:gut | IgG | 50 | 3/5 | 60 | 11, 47, 83 |
| Anti-Hh | 50 | 1/6 | 17 | 99 | ||
| E11 | AGM | IgG | 50 | 4/11 | 36 | >10, >10, >10, >10 |
| Anti-Hh | 50 | 3/12 | 25 | >10, >10, >10 |
| Embryonic day . | Tissue . | Factor or antibody . | Concentration (ng/ml) . | Number repopulated/number transplanted . | % Recipients repopulated . | % Chimerism . |
|---|---|---|---|---|---|---|
| Early E10 | AGM | Ihh | 2 | 0/9 | 0 | - |
| 20 | 4/10 | 40 | 100, 89, 99, 91 | |||
| 200 | 0/12 | 0 | - | |||
| Early E10 | AGM | Shh | 2 | 0/3 | 0 | - |
| 20 | 4/10 | 40 | 95, 89, 95, 33 | |||
| 200 | 0/8 | 0 | - | |||
| Early E10 | AGM:gut | IgG | 50 | 2/8 | 25 | 60, 99 |
| Anti-Hh | 50 | 0/7 | 0 | - | ||
| Late E10 | AGM:gut | IgG | 50 | 3/5 | 60 | 11, 47, 83 |
| Anti-Hh | 50 | 1/6 | 17 | 99 | ||
| E11 | AGM | IgG | 50 | 4/11 | 36 | >10, >10, >10, >10 |
| Anti-Hh | 50 | 3/12 | 25 | >10, >10, >10 |
E10 AGM explants (Ln72) were cultured for 3 days and cells transplanted into irradiated adult recipients (B10 × CBAF1). Recipients received 2-2.5 embryo equivalents of AGM cells. At >4 months after transplantation, peripheral blood DNA PCR was performed for the Ln72transgene. Percentage donor contribution was determined by comparison of the specific signal that was normalized for DNA content (myogenincontrol) with serial dilutions (100, 60, 30, 10, 6, 1 and 0%) of control transgenic DNA. Total number of experiments=14. For each condition n=2-4.