Skip to Main Content
Table 2.

HSC repopulation by chimeric reaggregates containing AGM and gut cells

Number of reaggregatesNumber of recipientsNumber of repopulated recipients (% chimerism)
E10 stageAGM markerGut markerAGM markerGut marker
Early IacZ Ln72 15 1 (43%) 
Early GFP Ln72 
Early Ln72 GFP 
Early Ln72 IacZ 3 (45%, 47%, 4%) 
Late IacZ Ln72 
Late GFP Ln72 
Late Ln72 GFP 1 (6%) 
Late Ln72 IacZ 3 (98%, 99%*, 100%
Number of reaggregatesNumber of recipientsNumber of repopulated recipients (% chimerism)
E10 stageAGM markerGut markerAGM markerGut marker
Early IacZ Ln72 15 1 (43%) 
Early GFP Ln72 
Early Ln72 GFP 
Early Ln72 IacZ 3 (45%, 47%, 4%) 
Late IacZ Ln72 
Late GFP Ln72 
Late Ln72 GFP 1 (6%) 
Late Ln72 IacZ 3 (98%, 99%*, 100%
Secondary transplantationNumber of BM cells/recipientNumber of recipientsNumber of repopulated recipients (% chimerism)
AGM markerGut markerAGM markerGut marker
*BM
 
Ln72
 
IacZ
 
2×106
 
5
 
5 (99%, 100%, 100%, 29%, 100%)
 
0
 
BM Ln72 IacZ 2×106 5 (68%, 53%, 83%, 58%, 60%) 
Secondary transplantationNumber of BM cells/recipientNumber of recipientsNumber of repopulated recipients (% chimerism)
AGM markerGut markerAGM markerGut marker
*BM
 
Ln72
 
IacZ
 
2×106
 
5
 
5 (99%, 100%, 100%, 29%, 100%)
 
0
 
BM Ln72 IacZ 2×106 5 (68%, 53%, 83%, 58%, 60%) 

Cells from E10 tissues from IacZ, Ln72 and GFP transgenic embryos were made into a chimeric reaggregate and cultured for 3 days. Harvested reaggregate cells were transplanted at a dose of 3 reaggregates(early E10) and 2 reaggregates (late E10) per adult irradiated recipient. At>4 months after transplantation, peripheral blood DNA PCR was performed with IacZ-, Ln72- and/or GFP-specific primers. Percentage donor contribution was determined by comparison of the specific signal that was normalized for DNA content (myogenin control) with serial dilutions (100, 60, 30, 10, 6, 1 and 0%) of control transgenic DNA. n=3.

*,†Individual primary recipient mice repopulated with cells derived from late E10 AGM (Ln72)/gut (IacZ)reaggregates. At the time of sacrifice, hematopoietic tissues were collected and checked by PCR for Ln72 and IacZ markers. Only Ln72 contribution was detected. Bone marrow (BM) of these two primary recipients was used for transplantation into five secondary recipients. At>4 months after transplantation, peripheral blood DNA PCR was performed with IacZ- and Ln72-specific primers.

Close Modal

or Create an Account

Close Modal
Close Modal