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Table 1.

CFU-S11 derived from chimeric reaggregates containing AGM and gut cells

E10 stageAGM markerGut markerNumber of reaggregatesNumber of recipientsNumber of CFU-S
Early GFP 11 8 GFP 0 GFP+ 
Early GFP 4 GFP+ 0 GFP 
Late GFP 4 GFP 0 GFP+ 
E10 stageAGM markerGut markerNumber of reaggregatesNumber of recipientsNumber of CFU-S
Early GFP 11 8 GFP 0 GFP+ 
Early GFP 4 GFP+ 0 GFP 
Late GFP 4 GFP 0 GFP+ 

Cells from E10 tissues from CAG-EGFP transgenic or nontransgenic embryos were made into chimeric reaggregates and cultured for 3 days. Harvested reaggregate cells were transplanted at a dose of 1.5-3 reaggregates(early E10) and 2 reaggregates (late E10) per adult irradiated recipient. Spleens were harvested at 11 days after injection and CFU-S11(colonies forming unit-spleen) were macroscopically counted under visible and fluorescent light. No CFU-S were found in noninjected irradiated control mice. DNA PCR verified the transgenic or nontransgenic genotype of the CFU-S11. n=3.

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