Skip to Main Content
Table 1.

Details of MRF and MyHC clones, and modifying enzymes used

Gene nameGenBank Accession no.Clone length (nt)PlasmidSense endonucleaseAntisense endonucleaseSense transcriptaseAntisense transcriptase
MyoD AB012882 1221 pBluescript SK- XhoSpeT3 T7 
Myogenin AB012881 855 pBluescript SK- NotXhoT7 T3 
Myf-5 AB012883 500 pBluescript SK- NotXhoT7 T3 
MyHC Eggs22 AJ009735 161 pBluescript+ EcoRI HindIII T3 T7 
MyHC Eggs24 AJ009734 152 pBluescript+ EcoRI HindIII T3 T7 
MyHC 10°C-type D50474 232 pBluescript SK- NotXhoT7 T3 
MyHC Intermediate-type D50475 523 pBluescript SK- NotXhoT7 T3 
MyHC 30°C-type D50476 254 pBluescript SK- NotXhoT7 T3 
Gene nameGenBank Accession no.Clone length (nt)PlasmidSense endonucleaseAntisense endonucleaseSense transcriptaseAntisense transcriptase
MyoD AB012882 1221 pBluescript SK- XhoSpeT3 T7 
Myogenin AB012881 855 pBluescript SK- NotXhoT7 T3 
Myf-5 AB012883 500 pBluescript SK- NotXhoT7 T3 
MyHC Eggs22 AJ009735 161 pBluescript+ EcoRI HindIII T3 T7 
MyHC Eggs24 AJ009734 152 pBluescript+ EcoRI HindIII T3 T7 
MyHC 10°C-type D50474 232 pBluescript SK- NotXhoT7 T3 
MyHC Intermediate-type D50475 523 pBluescript SK- NotXhoT7 T3 
MyHC 30°C-type D50476 254 pBluescript SK- NotXhoT7 T3 
Close Modal

or Create an Account

Close Modal
Close Modal