Details of MRF and MyHC clones, and modifying enzymes used
| Gene name . | GenBank Accession no. . | Clone length (nt) . | Plasmid . | Sense endonuclease . | Antisense endonuclease . | Sense transcriptase . | Antisense transcriptase . |
|---|---|---|---|---|---|---|---|
| MyoD | AB012882 | 1221 | pBluescript SK- | XhoI | SpeI | T3 | T7 |
| Myogenin | AB012881 | 855 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| Myf-5 | AB012883 | 500 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC Eggs22 | AJ009735 | 161 | pBluescript+ | EcoRI | HindIII | T3 | T7 |
| MyHC Eggs24 | AJ009734 | 152 | pBluescript+ | EcoRI | HindIII | T3 | T7 |
| MyHC 10°C-type | D50474 | 232 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC Intermediate-type | D50475 | 523 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC 30°C-type | D50476 | 254 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| Gene name . | GenBank Accession no. . | Clone length (nt) . | Plasmid . | Sense endonuclease . | Antisense endonuclease . | Sense transcriptase . | Antisense transcriptase . |
|---|---|---|---|---|---|---|---|
| MyoD | AB012882 | 1221 | pBluescript SK- | XhoI | SpeI | T3 | T7 |
| Myogenin | AB012881 | 855 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| Myf-5 | AB012883 | 500 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC Eggs22 | AJ009735 | 161 | pBluescript+ | EcoRI | HindIII | T3 | T7 |
| MyHC Eggs24 | AJ009734 | 152 | pBluescript+ | EcoRI | HindIII | T3 | T7 |
| MyHC 10°C-type | D50474 | 232 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC Intermediate-type | D50475 | 523 | pBluescript SK- | NotI | XhoI | T7 | T3 |
| MyHC 30°C-type | D50476 | 254 | pBluescript SK- | NotI | XhoI | T7 | T3 |