Oligonucleotide primers used to amplify α-subunit(Na+/K+-ATPase) and B-subunit (V-type H+-ATPase) cDNA from Dilocarcinus pagei gills
| Primer . | Nucleotide sequence (5′–3′) . |
|---|---|
| Na+/K+-ATPase | |
| Degenerate sense primer, NAK 10F | ATGACIGTIGCICAYATG |
| Degenerate antisense primer, NAK 16R | GGRTGRTCICCIGTIACCAT |
| V-type H+-ATPase | |
| Degenerate sense primer, HATF2 | GCNATGGGNGTNAAYATGGA |
| Degenerate antisense primer, HATR4 | TGNGTDATRTCRTCGTTNGG |
| Specific sense primer, DiloHATF1 | GTCGTCGTTGGGCATAGTAAGAAT |
| Specific antisense primer, DiloHATR1 | ATCCAACCATCGAACGTATCATCA |
| Primer . | Nucleotide sequence (5′–3′) . |
|---|---|
| Na+/K+-ATPase | |
| Degenerate sense primer, NAK 10F | ATGACIGTIGCICAYATG |
| Degenerate antisense primer, NAK 16R | GGRTGRTCICCIGTIACCAT |
| V-type H+-ATPase | |
| Degenerate sense primer, HATF2 | GCNATGGGNGTNAAYATGGA |
| Degenerate antisense primer, HATR4 | TGNGTDATRTCRTCGTTNGG |
| Specific sense primer, DiloHATF1 | GTCGTCGTTGGGCATAGTAAGAAT |
| Specific antisense primer, DiloHATR1 | ATCCAACCATCGAACGTATCATCA |
D, replaces A/G/T; N and I, replace A/C/G/T; R, replaces A/G; Y, replaces C/T.