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Table 1.

Differentially expressed proteins identified by 2D-DIGE/MS

GroupCG numberProtein descriptionMW (Da)pIMASCOT score
Group 1: upregulated at EW, WPP and 6AP 8091 Dronc, initiator caspase (Nedd2-like caspase) 51141 6.6 108 
 1803 Regucalcin* 33680 6.0 105 
 5261 Dihydrolipoyllysine-residue acetyltransferase 44118 8.9 149 
 9780 ATPase, Neprilysin 67766 9.1 72 
Group 2: downregulated at EW, WPP and 6AP 17285 Fat body protein 1 (FBP1) 119350 5.8 109 
 33102 Hexokinase-t1 (HEX-t1) 52918 6.1 67 
 3481 Alcohol dehydrogenase (ADH)* 27727 7.7 121 
 7176 Isocitrate dehydrolase (IDH)* 52955 7.2 205 
 16936 Glutathione transferase (GST) 25446 5.9 70 
 3752 Aldehyde dehydrogenase (ALDH)* 57325 6.4 122 
 6084 Aldehyde reductase 
 6180 Phosphatidylethanolamine binding 28706 9.0 420 
 17237 ATPase, Ca2+binding 21813 9.5 68 
 3092 Unknown 44565 6.3 99 
Group 3: upregulated at EW 12051 Actin 42A 41824 5.3 250 
 3922 Ribosomal protein S17 15332 10 77 
 1065 Succinyl coenzyme A synthatase, alpha subunit 34766 9.1 106 
Group 4: downregulated at WPP 3340 Krüppel (KR) 54715 7.1 120 
Group 5: upregulated at 6AP 4027 Actin 5C* 42194 5.3 96 
 14792 Stubarista (STA) 30266 4.8 78 
 7592 Odordant-binding protein 99b 17505 6.1 123 
GroupCG numberProtein descriptionMW (Da)pIMASCOT score
Group 1: upregulated at EW, WPP and 6AP 8091 Dronc, initiator caspase (Nedd2-like caspase) 51141 6.6 108 
 1803 Regucalcin* 33680 6.0 105 
 5261 Dihydrolipoyllysine-residue acetyltransferase 44118 8.9 149 
 9780 ATPase, Neprilysin 67766 9.1 72 
Group 2: downregulated at EW, WPP and 6AP 17285 Fat body protein 1 (FBP1) 119350 5.8 109 
 33102 Hexokinase-t1 (HEX-t1) 52918 6.1 67 
 3481 Alcohol dehydrogenase (ADH)* 27727 7.7 121 
 7176 Isocitrate dehydrolase (IDH)* 52955 7.2 205 
 16936 Glutathione transferase (GST) 25446 5.9 70 
 3752 Aldehyde dehydrogenase (ALDH)* 57325 6.4 122 
 6084 Aldehyde reductase 
 6180 Phosphatidylethanolamine binding 28706 9.0 420 
 17237 ATPase, Ca2+binding 21813 9.5 68 
 3092 Unknown 44565 6.3 99 
Group 3: upregulated at EW 12051 Actin 42A 41824 5.3 250 
 3922 Ribosomal protein S17 15332 10 77 
 1065 Succinyl coenzyme A synthatase, alpha subunit 34766 9.1 106 
Group 4: downregulated at WPP 3340 Krüppel (KR) 54715 7.1 120 
Group 5: upregulated at 6AP 4027 Actin 5C* 42194 5.3 96 
 14792 Stubarista (STA) 30266 4.8 78 
 7592 Odordant-binding protein 99b 17505 6.1 123 

Those proteins marked with an asterisk were detected twice, in two different protein spots. MASCOT scores >60 are significant(P<0.05).

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