Differentially expressed proteins identified by 2D-DIGE/MS
| Group . | CG number . | Protein description . | MW (Da) . | pI . | MASCOT score . |
|---|---|---|---|---|---|
| Group 1: upregulated at EW, WPP and 6AP | 8091 | Dronc, initiator caspase (Nedd2-like caspase) | 51141 | 6.6 | 108 |
| 1803 | Regucalcin* | 33680 | 6.0 | 105 | |
| 5261 | Dihydrolipoyllysine-residue acetyltransferase | 44118 | 8.9 | 149 | |
| 9780 | ATPase, Neprilysin | 67766 | 9.1 | 72 | |
| Group 2: downregulated at EW, WPP and 6AP | 17285 | Fat body protein 1 (FBP1) | 119350 | 5.8 | 109 |
| 33102 | Hexokinase-t1 (HEX-t1) | 52918 | 6.1 | 67 | |
| 3481 | Alcohol dehydrogenase (ADH)* | 27727 | 7.7 | 121 | |
| 7176 | Isocitrate dehydrolase (IDH)* | 52955 | 7.2 | 205 | |
| 16936 | Glutathione transferase (GST) | 25446 | 5.9 | 70 | |
| 3752 | Aldehyde dehydrogenase (ALDH)* | 57325 | 6.4 | 122 | |
| 6084 | Aldehyde reductase | - | - | - | |
| 6180 | Phosphatidylethanolamine binding | 28706 | 9.0 | 420 | |
| 17237 | ATPase, Ca2+binding | 21813 | 9.5 | 68 | |
| 3092 | Unknown | 44565 | 6.3 | 99 | |
| Group 3: upregulated at EW | 12051 | Actin 42A | 41824 | 5.3 | 250 |
| 3922 | Ribosomal protein S17 | 15332 | 10 | 77 | |
| 1065 | Succinyl coenzyme A synthatase, alpha subunit | 34766 | 9.1 | 106 | |
| Group 4: downregulated at WPP | 3340 | Krüppel (KR) | 54715 | 7.1 | 120 |
| Group 5: upregulated at 6AP | 4027 | Actin 5C* | 42194 | 5.3 | 96 |
| 14792 | Stubarista (STA) | 30266 | 4.8 | 78 | |
| 7592 | Odordant-binding protein 99b | 17505 | 6.1 | 123 |
| Group . | CG number . | Protein description . | MW (Da) . | pI . | MASCOT score . |
|---|---|---|---|---|---|
| Group 1: upregulated at EW, WPP and 6AP | 8091 | Dronc, initiator caspase (Nedd2-like caspase) | 51141 | 6.6 | 108 |
| 1803 | Regucalcin* | 33680 | 6.0 | 105 | |
| 5261 | Dihydrolipoyllysine-residue acetyltransferase | 44118 | 8.9 | 149 | |
| 9780 | ATPase, Neprilysin | 67766 | 9.1 | 72 | |
| Group 2: downregulated at EW, WPP and 6AP | 17285 | Fat body protein 1 (FBP1) | 119350 | 5.8 | 109 |
| 33102 | Hexokinase-t1 (HEX-t1) | 52918 | 6.1 | 67 | |
| 3481 | Alcohol dehydrogenase (ADH)* | 27727 | 7.7 | 121 | |
| 7176 | Isocitrate dehydrolase (IDH)* | 52955 | 7.2 | 205 | |
| 16936 | Glutathione transferase (GST) | 25446 | 5.9 | 70 | |
| 3752 | Aldehyde dehydrogenase (ALDH)* | 57325 | 6.4 | 122 | |
| 6084 | Aldehyde reductase | - | - | - | |
| 6180 | Phosphatidylethanolamine binding | 28706 | 9.0 | 420 | |
| 17237 | ATPase, Ca2+binding | 21813 | 9.5 | 68 | |
| 3092 | Unknown | 44565 | 6.3 | 99 | |
| Group 3: upregulated at EW | 12051 | Actin 42A | 41824 | 5.3 | 250 |
| 3922 | Ribosomal protein S17 | 15332 | 10 | 77 | |
| 1065 | Succinyl coenzyme A synthatase, alpha subunit | 34766 | 9.1 | 106 | |
| Group 4: downregulated at WPP | 3340 | Krüppel (KR) | 54715 | 7.1 | 120 |
| Group 5: upregulated at 6AP | 4027 | Actin 5C* | 42194 | 5.3 | 96 |
| 14792 | Stubarista (STA) | 30266 | 4.8 | 78 | |
| 7592 | Odordant-binding protein 99b | 17505 | 6.1 | 123 |
Those proteins marked with an asterisk were detected twice, in two different protein spots. MASCOT scores >60 are significant(P<0.05).