List of PCR primers used in the amplification of opsin sequences
| Primer . | Primer sequence 5′-3′ . |
|---|---|
| VSWS1F | TGGGCCTTYTACCTNCARRCNGCCTTYATGGG |
| VSWS1R | GCTCTTRGAGAARAASGCVGGGATGGTGAC |
| 5′ RACE adaptor primer | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG |
| P.borch SWS1F | TTATACAGTCGGAGGTCACGATGGGGAAG |
| P.borch SWS1R | CATCCTTTAATCAGTCGTCATTTTGTCCCATCG |
| D.mawsUVF | GCGCGAATTCCACCATGGGGAAGGACTTCCAC |
| D.mawsUVR | CGGCGTCGACGCAGACACTGAGGACACCTC |
| D.mawsGF | GCGCGAATTCCACCATGGAGACCAATGGCACAG |
| D.mawsGR | CGGCGTCGACGCAGACACAGAGGACACTTCTG |
| Blue400F | YYGTGGTCTCTKGCTGT |
| Blue818R | CCAGCATACCAAGAAGCC |
| T.loen3′I | GCAGAGTCTGCCTCCACC |
| MZbluestop | CTAWGCAGGYSCCYACTTTRG |
| T.loenF | CGGATGGAGCAGGTAC |
| T.loenR | GGTGGAGGCAGACTCTGC |
| Green1F | ATGAAYGGCTGARGGMAA |
| Green596R | CATATGATTCATTGTTGTAGCC |
| Green3′O | TCCAGATACATTCCTGAGGG |
| Green3′I | GCTGGAATTGGTGTTGGGA |
| GreenWkO | CTAGAAGCTTGAACATGATGGG |
| GreenWkI | TAAGGACTTCTAACAATCCC |
| UNI33 | (T)15GTTTGTTGT(G)7TT |
| UNI17 | TTTTTGTTTGTTGTGGG |
| Primer . | Primer sequence 5′-3′ . |
|---|---|
| VSWS1F | TGGGCCTTYTACCTNCARRCNGCCTTYATGGG |
| VSWS1R | GCTCTTRGAGAARAASGCVGGGATGGTGAC |
| 5′ RACE adaptor primer | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG |
| P.borch SWS1F | TTATACAGTCGGAGGTCACGATGGGGAAG |
| P.borch SWS1R | CATCCTTTAATCAGTCGTCATTTTGTCCCATCG |
| D.mawsUVF | GCGCGAATTCCACCATGGGGAAGGACTTCCAC |
| D.mawsUVR | CGGCGTCGACGCAGACACTGAGGACACCTC |
| D.mawsGF | GCGCGAATTCCACCATGGAGACCAATGGCACAG |
| D.mawsGR | CGGCGTCGACGCAGACACAGAGGACACTTCTG |
| Blue400F | YYGTGGTCTCTKGCTGT |
| Blue818R | CCAGCATACCAAGAAGCC |
| T.loen3′I | GCAGAGTCTGCCTCCACC |
| MZbluestop | CTAWGCAGGYSCCYACTTTRG |
| T.loenF | CGGATGGAGCAGGTAC |
| T.loenR | GGTGGAGGCAGACTCTGC |
| Green1F | ATGAAYGGCTGARGGMAA |
| Green596R | CATATGATTCATTGTTGTAGCC |
| Green3′O | TCCAGATACATTCCTGAGGG |
| Green3′I | GCTGGAATTGGTGTTGGGA |
| GreenWkO | CTAGAAGCTTGAACATGATGGG |
| GreenWkI | TAAGGACTTCTAACAATCCC |
| UNI33 | (T)15GTTTGTTGT(G)7TT |
| UNI17 | TTTTTGTTTGTTGTGGG |
Underlined bases identify EcoRI and SalI restriction enzyme sites for cloning into the expression vector pMT.