Haemodynamic effects of ET-1, ETB-agonist,ETA-antagonist and ET-1 before and after ETA-receptor blockade in the freshwater turtle, Trachemys scripta (Protocol 1)
| . | Psys (kPa) . | Q̇sys (ml min-1kg-1) . | Gsys (ml kPa-1 min-1kg-1) . | Ppul (kPa) . | Q̇pul (ml min-1kg-1) . | Gpul (ml kPa-1 min-1kg-1) . | fh (min-1) . | Q̇tot (ml min-1kg-1) . | Vstot (ml kg-1) . |
|---|---|---|---|---|---|---|---|---|---|
| Resting | 4.1±0.3 | 34.5±9.0 | 9.1±2.9 | 2.8±0.2 | 69.5±13.8 | 24.7±5.4 | 35.7±1.6 | 96.0±15.7 | 2.7±0.4 |
| ET-1 | 2.6±0.3* | 55.3±12.3* | 22.5±5.5* | 2.1±0.3* | 41.4±4.4* | 19.3±1.4 | 35.5±2.1 | 85.9±9.5 | 2.4±0.3 |
| Resting | 4.0±0.4 | 31.8±7.5 | 9.1±2.6 | 2.9±0.3 | 66.7±12.1 | 24.0±4.8 | 36.6±2.7 | 88.0±11.8 | 2.6±0.3 |
| ETB-agonist | 3.4±0.3* | 48.9±11.1* | 17.3±5.5* | 2.7±0.2 | 61.8±9.7 | 24.3±4.8 | 36.7±2.8 | 95.1±7.8 | 2.8±0.2 |
| Resting | 3.5±0.5 | 22.6±7.5 | 9.4±5.2 | 2.7±0.3 | 55.7±12.4 | 22.6±5.4 | 34.1±1.7 | 70.9±12.3 | 2.2±0.3 |
| ET-1 | 2.5±0.5* | 32.9±10.8* | 17.6±8.1* | 2.1±0.4 | 33.1±5.7 | 19.0±3.7 | 34.2±1.7 | 55.3±5.2 | 1.8±0.2 |
| Resting | 3.5±0.5 | 21.8±7.7 | 7.3±3.6 | 2.6±0.3 | 54.4±13.5 | 23.2±4.9 | 33.4±1.1 | 68.6±13.7 | 2.2±0.4 |
| ETA-antagonist | 3.6±0.5 | 23.8±8.2* | 7.7±3.3 | 2.6±0.4 | 54.2±14.1 | 22.5±5.0 | 33.0±1.2 | 70.0±14.8 | 2.2±0.4 |
| Resting | 3.6±0.5 | 23.9±8.4 | 7.8±3.3 | 2.5±0.4 | 53.7±14.6 | 22.6±5.4 | 32.8±1.1 | 69.5±15.3 | 2.2±0.5 |
| ET-1 | 2.9±0.5 | 31.3±9.8* | 12.1±4.0* | 2.2±0.4 | 39.0±8.1 | 20.5±4.1 | 32.7±1.3 | 60.7±9.4 | 2.0±0.3 |
| N | 7 | 7 | 7 | 5 | 6 | 5 | 7 | 6 | 6 |
| . | Psys (kPa) . | Q̇sys (ml min-1kg-1) . | Gsys (ml kPa-1 min-1kg-1) . | Ppul (kPa) . | Q̇pul (ml min-1kg-1) . | Gpul (ml kPa-1 min-1kg-1) . | fh (min-1) . | Q̇tot (ml min-1kg-1) . | Vstot (ml kg-1) . |
|---|---|---|---|---|---|---|---|---|---|
| Resting | 4.1±0.3 | 34.5±9.0 | 9.1±2.9 | 2.8±0.2 | 69.5±13.8 | 24.7±5.4 | 35.7±1.6 | 96.0±15.7 | 2.7±0.4 |
| ET-1 | 2.6±0.3* | 55.3±12.3* | 22.5±5.5* | 2.1±0.3* | 41.4±4.4* | 19.3±1.4 | 35.5±2.1 | 85.9±9.5 | 2.4±0.3 |
| Resting | 4.0±0.4 | 31.8±7.5 | 9.1±2.6 | 2.9±0.3 | 66.7±12.1 | 24.0±4.8 | 36.6±2.7 | 88.0±11.8 | 2.6±0.3 |
| ETB-agonist | 3.4±0.3* | 48.9±11.1* | 17.3±5.5* | 2.7±0.2 | 61.8±9.7 | 24.3±4.8 | 36.7±2.8 | 95.1±7.8 | 2.8±0.2 |
| Resting | 3.5±0.5 | 22.6±7.5 | 9.4±5.2 | 2.7±0.3 | 55.7±12.4 | 22.6±5.4 | 34.1±1.7 | 70.9±12.3 | 2.2±0.3 |
| ET-1 | 2.5±0.5* | 32.9±10.8* | 17.6±8.1* | 2.1±0.4 | 33.1±5.7 | 19.0±3.7 | 34.2±1.7 | 55.3±5.2 | 1.8±0.2 |
| Resting | 3.5±0.5 | 21.8±7.7 | 7.3±3.6 | 2.6±0.3 | 54.4±13.5 | 23.2±4.9 | 33.4±1.1 | 68.6±13.7 | 2.2±0.4 |
| ETA-antagonist | 3.6±0.5 | 23.8±8.2* | 7.7±3.3 | 2.6±0.4 | 54.2±14.1 | 22.5±5.0 | 33.0±1.2 | 70.0±14.8 | 2.2±0.4 |
| Resting | 3.6±0.5 | 23.9±8.4 | 7.8±3.3 | 2.5±0.4 | 53.7±14.6 | 22.6±5.4 | 32.8±1.1 | 69.5±15.3 | 2.2±0.5 |
| ET-1 | 2.9±0.5 | 31.3±9.8* | 12.1±4.0* | 2.2±0.4 | 39.0±8.1 | 20.5±4.1 | 32.7±1.3 | 60.7±9.4 | 2.0±0.3 |
| N | 7 | 7 | 7 | 5 | 6 | 5 | 7 | 6 | 6 |
Values are mean ± s.e.m.
Psys, mean systemic arterial pressure; Q̇sys, systemic blood flow; Gsys, systemic conductance; Ppul, mean pulmonary arterial pressure; Q̇pul, pulmonary blood flow; Gpul, pulmonary conductance; fh, heart rate; Q̇tot, total cardiac output; Vstot, total stroke volume.
ET-1, endothelin-1 (120 pmol kg-1); ETB-agonist (150 pmol kg-1); ETA-antagonist (0.15 μmol kg-1).
denotes a significant difference from resting values(P<0.05)