RNA . | Dose (pg) . | Normal (%) . | Inter-proneuronal domain-specific contribution*(%) . | Reduced expression†(%) . | Ectopic and spotty expression‡(%) . | n . |
---|---|---|---|---|---|---|
β-gal only | 50 | 100 | 0 | 0 | 0 | 38 |
Her9MT | 5 | 18 | 67 | 15 | 0 | 139 |
20 | 13 | 79 | 8 | 0 | 78 | |
Her9EnR | 2 | 10 | 78 | 12 | 0 | 46 |
10 | 9 | 83 | 8 | 0 | 33 | |
Her9VP16 | 5 | 24 | 0 | 2 | 74 | 116 |
20 | 17 | 0 | 3 | 80 | 39 | |
Her3MT | 20 | 61 | 6 | 33 | 0 | 94 |
100 | 44 | 8 | 46 | 0 | 69 |
RNA . | Dose (pg) . | Normal (%) . | Inter-proneuronal domain-specific contribution*(%) . | Reduced expression†(%) . | Ectopic and spotty expression‡(%) . | n . |
---|---|---|---|---|---|---|
β-gal only | 50 | 100 | 0 | 0 | 0 | 38 |
Her9MT | 5 | 18 | 67 | 15 | 0 | 139 |
20 | 13 | 79 | 8 | 0 | 78 | |
Her9EnR | 2 | 10 | 78 | 12 | 0 | 46 |
10 | 9 | 83 | 8 | 0 | 33 | |
Her9VP16 | 5 | 24 | 0 | 2 | 74 | 116 |
20 | 17 | 0 | 3 | 80 | 39 | |
Her3MT | 20 | 61 | 6 | 33 | 0 | 94 |
100 | 44 | 8 | 46 | 0 | 69 |
RNAs for Her9MT, Her9VP16, Her9EnR or Her3MT with β-galactosidase(β-gal) RNA were co-injected into one blastomere of two- to four-cell stage embryos, and the embryos were fixed at the three-somite stage and stained with X-gal and the neurod4 riboprobe. The embryos were classified into four categories by the expression of neurod4 and the contribution of the blue cells, which received the exogenous RNA.
Embryos had the blue cells only in the inter-proneuronal domains(Fig. 5O).
Embryos showed reduced or abolished expression of neurod4 in the proneuronal domains (Fig. 5P,R).
Embryos showed ectopic and spotty expression of neurod4 in the neural plate (Fig. 5Q). The embryos that did not show any changes in the neurod4 expression or inter-proneuronal domain-specific contribution of the blue cells were classified as `normal'. The embryos with blue cells in the ventral epidermis or the mesoderm were also classified as `normal'.