Pathfinding defects of OSN axons in 3.5-dpf embryos
| . | . | Posterior pathfinding errors . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Maximal reach*(μm) . | . | . | . | ||||
| . | n . | No obvious pathfinding errors . | <100 . | 100-200 . | >200 . | Midline-crossing errors . | ||||
| Wild type | 13 | 13 | 0 | 0 | 0 | 0 | ||||
| ast/+ | 12 | 12 | 0 | 0 | 0 | 0 | ||||
| ast/ast | 13 | 0 | 3 | 8 | 2 | 7 | ||||
| wt hs† | 12 | 12 | 0 | 0 | 0 | 0 | ||||
| slit2 no-hs‡ | 10 | 9 | 1 | 0 | 0 | 0 | ||||
| slit2 hs§ | 12 | 0 | 2 | 9 | 1 | 8 | ||||
| . | . | Posterior pathfinding errors . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Maximal reach*(μm) . | . | . | . | ||||
| . | n . | No obvious pathfinding errors . | <100 . | 100-200 . | >200 . | Midline-crossing errors . | ||||
| Wild type | 13 | 13 | 0 | 0 | 0 | 0 | ||||
| ast/+ | 12 | 12 | 0 | 0 | 0 | 0 | ||||
| ast/ast | 13 | 0 | 3 | 8 | 2 | 7 | ||||
| wt hs† | 12 | 12 | 0 | 0 | 0 | 0 | ||||
| slit2 no-hs‡ | 10 | 9 | 1 | 0 | 0 | 0 | ||||
| slit2 hs§ | 12 | 0 | 2 | 9 | 1 | 8 | ||||
OSN axons were labeled by external application of DiI at 3.5 dpf. Numbers indicate the number of embryos showing a given axon pathfinding error.
The distance from the boundary between the olfactory epithelium and the telencephalon to the farthest point of the posterior misrouted axons, measured from composite dorsal view images from a series of optical sections.
Heat-induced wild-type embryos.
Non-heat-induced hsp:slit2-gfp/+ embryos.
Heat-induced hsp:slit2-gfp/+ embryos.