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Table 1.

Pathfinding defects of OSN axons in 3.5-dpf embryos

Posterior pathfinding errors
Maximal reach*(μm)
nNo obvious pathfinding errors<100100-200>200Midline-crossing errors
Wild type 13 13 
ast/+ 12 12 
ast/ast 13 
wt hs 12 12 
slit2 no-hs 10 
slit2 hs§ 12 
Posterior pathfinding errors
Maximal reach*(μm)
nNo obvious pathfinding errors<100100-200>200Midline-crossing errors
Wild type 13 13 
ast/+ 12 12 
ast/ast 13 
wt hs 12 12 
slit2 no-hs 10 
slit2 hs§ 12 

OSN axons were labeled by external application of DiI at 3.5 dpf. Numbers indicate the number of embryos showing a given axon pathfinding error.

*

The distance from the boundary between the olfactory epithelium and the telencephalon to the farthest point of the posterior misrouted axons, measured from composite dorsal view images from a series of optical sections.

Heat-induced wild-type embryos.

Non-heat-induced hsp:slit2-gfp/+ embryos.

§

Heat-induced hsp:slit2-gfp/+ embryos.

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