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Table 1.

Mutations in alr-1 affect gene expression in the AWA and ASG chemosensory neurons

% of sides showing expression in neuron number:
Neuron typeMarker*Genotype102
AWA odr-7::gfp Wild type 100 
  alr-1(oy42) 57 35 
  alr-1(oy56) 71 22 
  alr-1(oy42);Ex[alr-1 genomic96 
  alr-1(oy56);Ex[alr-1 genomic98 
  alr-1(oy42);Ex[alr-1p::alr-1 cDNA] 87 
  alr-1(oy42);Ex[alr-1p::Al cDNA] 93 
 ODR-7 Wild type 94 
  alr-1(oy42) 66 28 
  alr-1(oy56) 81 12 
  alr-1(ok545) 78 13 
 odr-10::gfp Wild type 100 <1 
  alr-1(oy42) 73 22 
  alr-1(oy56) 82 10 
ASG ops-1::dsRed Wild type 100 <1 
  alr-1(oy42) 81 19 <1 
  alr-1(oy56) 89 11 <1 
  alr-1(ok545) 87 13 
  alr-1(oy42);Ex[alr-1 genomic98 
  alr-1(oy42);Ex[alr-1p::alr-1 cDNA] 98 
  alr-1(oy42);Ex[alr-1p::Al cDNA] 93 
 Ex[unc-30::gfpWild type 76 24 
  alr-1(oy42) 37 63 
  alr-1(oy56) 61 39 
% of sides showing expression in neuron number:
Neuron typeMarker*Genotype102
AWA odr-7::gfp Wild type 100 
  alr-1(oy42) 57 35 
  alr-1(oy56) 71 22 
  alr-1(oy42);Ex[alr-1 genomic96 
  alr-1(oy56);Ex[alr-1 genomic98 
  alr-1(oy42);Ex[alr-1p::alr-1 cDNA] 87 
  alr-1(oy42);Ex[alr-1p::Al cDNA] 93 
 ODR-7 Wild type 94 
  alr-1(oy42) 66 28 
  alr-1(oy56) 81 12 
  alr-1(ok545) 78 13 
 odr-10::gfp Wild type 100 <1 
  alr-1(oy42) 73 22 
  alr-1(oy56) 82 10 
ASG ops-1::dsRed Wild type 100 <1 
  alr-1(oy42) 81 19 <1 
  alr-1(oy56) 89 11 <1 
  alr-1(ok545) 87 13 
  alr-1(oy42);Ex[alr-1 genomic98 
  alr-1(oy42);Ex[alr-1p::alr-1 cDNA] 98 
  alr-1(oy42);Ex[alr-1p::Al cDNA] 93 
 Ex[unc-30::gfpWild type 76 24 
  alr-1(oy42) 37 63 
  alr-1(oy56) 61 39 

Adult animals grown at 25°C were examined. n>100 for each.

*

ODR-7 expression was examined by staining with anti-ODR-7 antibodies(Sarafi-Reinach et al., 2001). The odr-7::gfp (Sengupta et al.,1994), odr-10::gfp(Sengupta et al., 1996) and ops-1::dsRed (Sarafi-Reinach et al., 2001) transgenes were stably integrated into the genome. unc-30::gfp (Jin et al.,1994) was present on extrachromosomal arrays, and expression from the same arrays was examined in wild-type and mutant backgrounds. Numbers shown are from strains carrying two different unc-30::gfp arrays,with the exception of alr-1(oy56) in which expression from a single array was examined

For extrachromosomal arrays, numbers shown are from at least two independent arrays examined in a given strain background

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