Table 1.

Novel primer sets used in this work

Oligonucleotide sequences (5′→3′)
PurposeMarker/geneBACPosition in BACForward primer (label, if any)Reverse primer
Linkage analysis ve012 T8K22 60,467 CGATGGGTTCCATCGAAGAA CAGCATACAATTTCACTTCC 
 nga1145 T8K22 69,343 CCTTCACATCCAAAACCCAC (HEX) GCACATACCCACAACCAGAA 
 T8K22-4 T8K22 60,526 TCTTCCTCCATATTGTCCGT CCGAGACTTGAGTGGTAGCT 
 P450 T8K22 49,031 GCTCTATCCAGATGTACTAC AGCCATCTTAATGGTTAGTA 
 T8K22-7 T8K22 46,066 GCATTAGTGTAAAAGCCCATTAC TGTCCCGTTACTCAGTAGAAAG 
 T8K22-1 T8K22 7511 CAGATACAGCTATGTACGGT GTTGAACTCCTCCGTTGAGA 
 E2-SNP T20F6 29,254 CTACGATGATTCGTTGCTAG ACGAGATTCGAGACTCGGTA 
 T17M13b T17M13 67,337 GCTATTGATTCCATGATCCCTA GTCGGTGGATGAGCACAAGATA 
 m246 F19B11 52,017 TGAAGAGCTATCCGAGATGG GCTTGAACTCCTCCTCCTTC 
Genomic DNA amplification and cycle sequencing HVE T8K22 61,139 TGAGATCCTTGTTTGATTGCAG AAGAAGGAAGATAGGAAGTACC* 
 HVE T8K22 60,077 GTCAGCTTCTCTTGCTTCCAG CCTCCCTTTCCTTAAATCTATC 
 HVE T8K22 59,131 CATGACGGGAGTATACAAGATT CGTACTACTCTGACAAGTTCC† 
 HVE T8K22 57,981 TCCCTACACTTCAAAACCCAG GAAAAAACACAGACGAAGTCATC 
 HVE T8K22 56,848 TCTTGTGGAGCTTTCATCTCTG TCTACCTGAAGTGCTGGAACAA 
 HVE T8K22 55,505 ACCACTGTGAAAGCGAGGAAG* ATTCTTCTTCGCCGATTGAGAAA* 
 HVE T8K22 60,279 TGCTCACTAAAGTATATCAAGAG†  
 HVE T8K22 62,123 CAGCATCGTGATACCGCAAG*  
Oligonucleotide sequences (5′→3′)
PurposeMarker/geneBACPosition in BACForward primer (label, if any)Reverse primer
Linkage analysis ve012 T8K22 60,467 CGATGGGTTCCATCGAAGAA CAGCATACAATTTCACTTCC 
 nga1145 T8K22 69,343 CCTTCACATCCAAAACCCAC (HEX) GCACATACCCACAACCAGAA 
 T8K22-4 T8K22 60,526 TCTTCCTCCATATTGTCCGT CCGAGACTTGAGTGGTAGCT 
 P450 T8K22 49,031 GCTCTATCCAGATGTACTAC AGCCATCTTAATGGTTAGTA 
 T8K22-7 T8K22 46,066 GCATTAGTGTAAAAGCCCATTAC TGTCCCGTTACTCAGTAGAAAG 
 T8K22-1 T8K22 7511 CAGATACAGCTATGTACGGT GTTGAACTCCTCCGTTGAGA 
 E2-SNP T20F6 29,254 CTACGATGATTCGTTGCTAG ACGAGATTCGAGACTCGGTA 
 T17M13b T17M13 67,337 GCTATTGATTCCATGATCCCTA GTCGGTGGATGAGCACAAGATA 
 m246 F19B11 52,017 TGAAGAGCTATCCGAGATGG GCTTGAACTCCTCCTCCTTC 
Genomic DNA amplification and cycle sequencing HVE T8K22 61,139 TGAGATCCTTGTTTGATTGCAG AAGAAGGAAGATAGGAAGTACC* 
 HVE T8K22 60,077 GTCAGCTTCTCTTGCTTCCAG CCTCCCTTTCCTTAAATCTATC 
 HVE T8K22 59,131 CATGACGGGAGTATACAAGATT CGTACTACTCTGACAAGTTCC† 
 HVE T8K22 57,981 TCCCTACACTTCAAAACCCAG GAAAAAACACAGACGAAGTCATC 
 HVE T8K22 56,848 TCTTGTGGAGCTTTCATCTCTG TCTACCTGAAGTGCTGGAACAA 
 HVE T8K22 55,505 ACCACTGTGAAAGCGAGGAAG* ATTCTTCTTCGCCGATTGAGAAA* 
 HVE T8K22 60,279 TGCTCACTAAAGTATATCAAGAG†  
 HVE T8K22 62,123 CAGCATCGTGATACCGCAAG*  
*

Oligonucleotides used together with LBb1 and LBa1(http://signal.salk.edu)for ascertaining the presence and position of T-DNA insertions in the N599479 and N610969 lines.

†

Oligonucleotides used in amplifications of HVE cDNA.

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