Oligonucleotides used in RT-PCR
| Gene . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| Epcam | CGGCTCAGAGAGACTGTGTC | GATCCAGTAGGTCCTCACGC |
| Ck19 | GTCCTACAGATTGACAATG | CACGCTCTGGATCTGTGACAG |
| Ck7 | GGGATGACCTCCGCAACACC | CTCCAGCAGCTTGCGGTAGG |
| Alb | CATGACACCATGCCTGCTGAT | GCCTTTCCACCAGGGATCCAC |
| Afp | CTGGAGTGTCTGCAGGATGG | CCACAGCCGGACCATTTCTC |
| Gapdh | GGAGCGAGACCCCACTAA | GTGTAGCCCAAGATGCCC |
| Hprt | GACTGAAAGACTTGCTCGAG | CCAGCAAGCTTGCAACCTTAACAA |
| G6Pase | AACCCATTGTGAGGCCAGAGG | TACTCATTACACTAGTTGGTC |
| Tat | TGCATCCTCCTGAAGACATG | CTTCTCTCTGGTGTAGCTCT |
| Cps | ACTGAGAGATGCTGACCCTA | CCTGGAAATTGGTGAGGAGA |
| integrin β4 | GACCTATGAAGAAGGTGCTC | GGCTCAGATGCGTGCCATAG |
| Ggt1 | GCTGAGCTGATTGAGCATCCG | GGTTGATGAAGTTGGGCGAGC |
| mucin 1 | GAGCGCCAGCCTTGAGTTTG | GGAGGCACTACTGTGGACTG |
| Trop2 | CTGACCTAGACTCCGAGCTG | CCAACCCATCTGGTCTGAGG |
| claudin 4 | GACTTTGACCCCTGCAGAGG | GGCCACAGGCTGTTATGAGC |
| Cd44 | CAGAGGCGACTAGATCCCTC | GAGTCACAGTGCGGGAACTC |
| Slc10a1 | AGATCAAGGCTCACTTCTGG | AGAAGTCCTTCTGCAAGCTG |
| Tdo2 | ACAATGAAGAAGACAGAGC | TGTAGTCTCCTCCAAAGTTA |
| Gene . | Forward (5′ to 3′) . | Reverse (5′ to 3′) . |
|---|---|---|
| Epcam | CGGCTCAGAGAGACTGTGTC | GATCCAGTAGGTCCTCACGC |
| Ck19 | GTCCTACAGATTGACAATG | CACGCTCTGGATCTGTGACAG |
| Ck7 | GGGATGACCTCCGCAACACC | CTCCAGCAGCTTGCGGTAGG |
| Alb | CATGACACCATGCCTGCTGAT | GCCTTTCCACCAGGGATCCAC |
| Afp | CTGGAGTGTCTGCAGGATGG | CCACAGCCGGACCATTTCTC |
| Gapdh | GGAGCGAGACCCCACTAA | GTGTAGCCCAAGATGCCC |
| Hprt | GACTGAAAGACTTGCTCGAG | CCAGCAAGCTTGCAACCTTAACAA |
| G6Pase | AACCCATTGTGAGGCCAGAGG | TACTCATTACACTAGTTGGTC |
| Tat | TGCATCCTCCTGAAGACATG | CTTCTCTCTGGTGTAGCTCT |
| Cps | ACTGAGAGATGCTGACCCTA | CCTGGAAATTGGTGAGGAGA |
| integrin β4 | GACCTATGAAGAAGGTGCTC | GGCTCAGATGCGTGCCATAG |
| Ggt1 | GCTGAGCTGATTGAGCATCCG | GGTTGATGAAGTTGGGCGAGC |
| mucin 1 | GAGCGCCAGCCTTGAGTTTG | GGAGGCACTACTGTGGACTG |
| Trop2 | CTGACCTAGACTCCGAGCTG | CCAACCCATCTGGTCTGAGG |
| claudin 4 | GACTTTGACCCCTGCAGAGG | GGCCACAGGCTGTTATGAGC |
| Cd44 | CAGAGGCGACTAGATCCCTC | GAGTCACAGTGCGGGAACTC |
| Slc10a1 | AGATCAAGGCTCACTTCTGG | AGAAGTCCTTCTGCAAGCTG |
| Tdo2 | ACAATGAAGAAGACAGAGC | TGTAGTCTCCTCCAAAGTTA |