Table 2.

Effect of temperature change on expression levels of crustacean cardioactive peptide and arginine kinase mRNA in juvenile Callinectes sapidus

Copy no per eyestalk
TreatmentCCAP (×105)AK (×107)
22 to 4°C 4.83±0.95 10.7±3.49 
22 to 22°C 6.15±0.82 8.04±1.92 
22 to 29°C 9.74±1.25 16.0±2.78 
Copy no per eyestalk
TreatmentCCAP (×105)AK (×107)
22 to 4°C 4.83±0.95 10.7±3.49 
22 to 22°C 6.15±0.82 8.04±1.92 
22 to 29°C 9.74±1.25 16.0±2.78 

CCAP, crustacean cardioactive peptide; AK, Arginine kinase.

Crabs were exposed to hypothermal stress (22°C to 4°C),hyperthermal stress (22°C to 29°C), or kept at 22°C, for 2 h,followed by rapid extraction and purification of eyestalk tissues. Values are means ± 1 s.e.m. (N=6).

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