Primer sequences used for crustacean cardioactive peptide sequence identification and quantitative PCR
| Primer . | Sequence . | |
|---|---|---|
| (A) Crustacean cardioactive peptide (CCAP) | ||
| Degenerate primers | ||
| dF1 | AARMGICCNTTYTGYAAYGC | |
| dF2 | CCITTYTGYAAYGCNTTYAC | |
| dF3 | TGYAAYGCITTYACNGGITG | |
| Carcinus maenas GSP | ||
| CCAP5′R1 | GCCAGAAGCAAGTCCTTCTAGCTCAGGG | |
| CCAP5′R2 | CTCAGGGTCAGACCGCTTCTTACCAC | |
| CCAP1F | GTTGGGACGTACATGGCTGGTG | |
| CCAP1R | GTCGGCTGTGTTTTCTGGTCTTCA | |
| CCAP2F | AGCCTTCTCCTTCTGGTGTTT | |
| CCAP2R | CATCTTGCTTTGGAGTTGTTC | |
| Callinectes sapidus GSP | ||
| CCAP5′R1 | GCAAGGCCCTCCAGCTCAGGATCGGA | |
| CCAP5′R2 | CTCAGGATCGGATCGTTTCTTGCCGC | |
| CCAPLF | ATCCTCCTTCTGGCATTCCT | |
| CCAPLR | CCGCTATGTCTCCTGCTGTT | |
| CCAPSF | AAACACTGAAGCAGGGATCG | |
| CCAPSR | AGCTCTGAGCCAGAAGCAAG | |
| Homarus gammarus GSP | ||
| CCAP5′R1 | GAGGCCAAGCCTTCCATGCTGGGGTCTG | |
| CCAP5′R2 | CTTCCATGCTGGGGTCTGACCTCTT | |
| Orconectes immunis GSP | ||
| CCAP5′R1 | GAAGCCACGCCCTCCAGGCCGGGGTCTG | |
| CCAP5′R2 | CCTCCAGGCCGGGGTCTGACCGCTT | |
| (B) Arginine kinase (AK) | ||
| Carcinus maenas AK (accession no. AF167313) | ||
| AKSF | AAACGGTCACCCTCCTTGA | |
| AKLF | AAAGGTTTCCTCCACCCTGT | |
| AKLR | ACTTCCTCGAGCTTGTCACG | |
| Callinectes sapidus AK (accession no. Q9NH49) | ||
| AKLF | GACCCCATCATCGAGGACTA | |
| AKLR | CCACACCAGGAAGGTCTTGT | |
| AKSF | ACCACAAGGGTTTCAAGCAG | |
| AKSR | GGTGGAGGAAACCTTGGAC | |
| Primer . | Sequence . | |
|---|---|---|
| (A) Crustacean cardioactive peptide (CCAP) | ||
| Degenerate primers | ||
| dF1 | AARMGICCNTTYTGYAAYGC | |
| dF2 | CCITTYTGYAAYGCNTTYAC | |
| dF3 | TGYAAYGCITTYACNGGITG | |
| Carcinus maenas GSP | ||
| CCAP5′R1 | GCCAGAAGCAAGTCCTTCTAGCTCAGGG | |
| CCAP5′R2 | CTCAGGGTCAGACCGCTTCTTACCAC | |
| CCAP1F | GTTGGGACGTACATGGCTGGTG | |
| CCAP1R | GTCGGCTGTGTTTTCTGGTCTTCA | |
| CCAP2F | AGCCTTCTCCTTCTGGTGTTT | |
| CCAP2R | CATCTTGCTTTGGAGTTGTTC | |
| Callinectes sapidus GSP | ||
| CCAP5′R1 | GCAAGGCCCTCCAGCTCAGGATCGGA | |
| CCAP5′R2 | CTCAGGATCGGATCGTTTCTTGCCGC | |
| CCAPLF | ATCCTCCTTCTGGCATTCCT | |
| CCAPLR | CCGCTATGTCTCCTGCTGTT | |
| CCAPSF | AAACACTGAAGCAGGGATCG | |
| CCAPSR | AGCTCTGAGCCAGAAGCAAG | |
| Homarus gammarus GSP | ||
| CCAP5′R1 | GAGGCCAAGCCTTCCATGCTGGGGTCTG | |
| CCAP5′R2 | CTTCCATGCTGGGGTCTGACCTCTT | |
| Orconectes immunis GSP | ||
| CCAP5′R1 | GAAGCCACGCCCTCCAGGCCGGGGTCTG | |
| CCAP5′R2 | CCTCCAGGCCGGGGTCTGACCGCTT | |
| (B) Arginine kinase (AK) | ||
| Carcinus maenas AK (accession no. AF167313) | ||
| AKSF | AAACGGTCACCCTCCTTGA | |
| AKLF | AAAGGTTTCCTCCACCCTGT | |
| AKLR | ACTTCCTCGAGCTTGTCACG | |
| Callinectes sapidus AK (accession no. Q9NH49) | ||
| AKLF | GACCCCATCATCGAGGACTA | |
| AKLR | CCACACCAGGAAGGTCTTGT | |
| AKSF | ACCACAAGGGTTTCAAGCAG | |
| AKSR | GGTGGAGGAAACCTTGGAC | |
GSP, gene-specific primers.