Table 4.

Fluid flow in Kupffer's vesicle of sea mutants

GenotypenStrong flowReduced flowAbsent flow
seatg238a (+/+, +/–) 3 (1) 1 (1) – 
seatg238 (–/–) 1 (1) 1 (0) 3 (1) 
seafa20r (+/+, +/–) 8 (6) – – 
seafa20r (–/–) 5 (4) 1 (1) 2 (1) 
GenotypenStrong flowReduced flowAbsent flow
seatg238a (+/+, +/–) 3 (1) 1 (1) – 
seatg238 (–/–) 1 (1) 1 (0) 3 (1) 
seafa20r (+/+, +/–) 8 (6) – – 
seafa20r (–/–) 5 (4) 1 (1) 2 (1) 

The tg238a/+ pair used to produce the embryos for this experiment(tg238a-C) have a high degree of left-right patterning defects (28%). The fa20r/+ pair used to produce the embryos for this experiment(fa20r-M21) have a low degree of left-right patterning defects (2%). See Table S1 for details. Numbers in parentheses indicate embryos with correct heart looping in each category

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