Primers used in this study
| Primer . | Sequence (5′ to 3′) . |
|---|---|
| AuxRE mutagenesis | |
| Mut ARE-A | GAGAGATTAGGAGCCATTTGATCAGAGC |
| check mut-a | AGAGTAATAGTAATAAAAGAGTAGTC |
| Mut ARE-B | TAATTGCTGCTTATTGGCTCATTCAGTCATTTGC |
| check mut-b | CTATGGTTTCACATAGAGTTGTAACATTTC |
| Mut ARE-C | CATGAAATACATATATGGCTCATACATACGTTTAG |
| check mut-c | GTAAGCATCGGGCGATATGGCATGATTATTTC |
| Mut ARE-D | CAAAAGGCAGTGTTTGGCTCTGTCTTCTCCAAC |
| check mut-d | GGAAAATTCCTTTGTTCAGCAATGTTGGTATC |
| Mut ARE-E | GTAATTTAATCTACTGGCTCATATAAGTAGACAAC |
| check mut-e | GTATGTTTCGTGGTTATTGCGTTGATGTAAC |
| ChIP analysis | |
| RTnonAREf | CCATGGTGGTTTTGGTCTTTAC |
| RTnonAREr | TGTATTCAAATTTGTTTGGTTGG |
| RT-ARE-Af | TGCATACATATGAATCTGACCCTA |
| RT-ARE-Ar | GAAGAGGAGCAAAATGATCTGC |
| RT-ARE-Bf | CAGAATTAACGCAGGGGTTTTA |
| RT-ARE-Br | AAACCATCTACCATGACCATGAC |
| RT-ARE-Cf | AACAAGCTTCTGATACATGAAATACA |
| RT-ARE-Cr | CGAAGAGGACATTTCAGGTTTG |
| RT-ORFf | AACTCTTTCAACGGCTCATCAT |
| RT-ORFr | ACGACTCGTTGTTTTCGTTTTC |
| RT-actin2f | GTGTTGTTAGCAACTGGGATGA |
| RT-actin2r | CTCTTCAGGAGCAATACGAAGC |
| Genotyping | |
| ARF5f | GGCTATTGAGATATTTTGGC |
| ARF5r (+LBb1) | CATTGAGGATTGAAGAAGCT |
| MP-S319f | GATTTTCCAGATAATTCTGGA |
| MP-S319r (+LBa1) | ATGAATATAGTTTCAGGTCTC |
| DRNf (+SPM8) | ATGGAAAAAGCCTTGAGAAAC |
| DRNr | ATCATGATGAATATCTAGCTA |
| LBa1salk T-DNA border | TGGTTCACGTAGTGGGCCATCG |
| LBb1salk T-DNA border | GCGTGGACCGCTTGCTGCAACT |
| Spm8 | GTTTTGGCCGACACTCCTTAC |
| Primer . | Sequence (5′ to 3′) . |
|---|---|
| AuxRE mutagenesis | |
| Mut ARE-A | GAGAGATTAGGAGCCATTTGATCAGAGC |
| check mut-a | AGAGTAATAGTAATAAAAGAGTAGTC |
| Mut ARE-B | TAATTGCTGCTTATTGGCTCATTCAGTCATTTGC |
| check mut-b | CTATGGTTTCACATAGAGTTGTAACATTTC |
| Mut ARE-C | CATGAAATACATATATGGCTCATACATACGTTTAG |
| check mut-c | GTAAGCATCGGGCGATATGGCATGATTATTTC |
| Mut ARE-D | CAAAAGGCAGTGTTTGGCTCTGTCTTCTCCAAC |
| check mut-d | GGAAAATTCCTTTGTTCAGCAATGTTGGTATC |
| Mut ARE-E | GTAATTTAATCTACTGGCTCATATAAGTAGACAAC |
| check mut-e | GTATGTTTCGTGGTTATTGCGTTGATGTAAC |
| ChIP analysis | |
| RTnonAREf | CCATGGTGGTTTTGGTCTTTAC |
| RTnonAREr | TGTATTCAAATTTGTTTGGTTGG |
| RT-ARE-Af | TGCATACATATGAATCTGACCCTA |
| RT-ARE-Ar | GAAGAGGAGCAAAATGATCTGC |
| RT-ARE-Bf | CAGAATTAACGCAGGGGTTTTA |
| RT-ARE-Br | AAACCATCTACCATGACCATGAC |
| RT-ARE-Cf | AACAAGCTTCTGATACATGAAATACA |
| RT-ARE-Cr | CGAAGAGGACATTTCAGGTTTG |
| RT-ORFf | AACTCTTTCAACGGCTCATCAT |
| RT-ORFr | ACGACTCGTTGTTTTCGTTTTC |
| RT-actin2f | GTGTTGTTAGCAACTGGGATGA |
| RT-actin2r | CTCTTCAGGAGCAATACGAAGC |
| Genotyping | |
| ARF5f | GGCTATTGAGATATTTTGGC |
| ARF5r (+LBb1) | CATTGAGGATTGAAGAAGCT |
| MP-S319f | GATTTTCCAGATAATTCTGGA |
| MP-S319r (+LBa1) | ATGAATATAGTTTCAGGTCTC |
| DRNf (+SPM8) | ATGGAAAAAGCCTTGAGAAAC |
| DRNr | ATCATGATGAATATCTAGCTA |
| LBa1salk T-DNA border | TGGTTCACGTAGTGGGCCATCG |
| LBb1salk T-DNA border | GCGTGGACCGCTTGCTGCAACT |
| Spm8 | GTTTTGGCCGACACTCCTTAC |