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Table 3.

Time course of the apparent affinity for ADP (K_m,app) and the maximal velocity (V_max) for oxygen consumption by mitochondria during warm acclimation

	5°C			15°C
	K_m	V_max	K_m	V_max
Cold acclimated	0.12±0.007^a	72.5±5.4^a	0.06±0.003^a	69.2±8.1^a
Short-term warm exposed	0.07±0.006^b	72.1±2.7^a	0.097±0.016^b	15.6±1.1^b
Warm acclimated	0.03±0.003^c	31.6±5.1^b	0.015±0.002^c	21.8±2.2^b

	5°C			15°C
	K_m	V_max	K_m	V_max
Cold acclimated	0.12±0.007^a	72.5±5.4^a	0.06±0.003^a	69.2±8.1^a
Short-term warm exposed	0.07±0.006^b	72.1±2.7^a	0.097±0.016^b	15.6±1.1^b
Warm acclimated	0.03±0.003^c	31.6±5.1^b	0.015±0.002^c	21.8±2.2^b

Values are means ± s.e.m. (N=8 for cold acclimated, N=7 for short term warm exposed and N=12 for warm-acclimated trout). Different superscript letters indicate rates at a given assay temperature that differ with acclimation state (ANOVA and a posteriori tests, P≤0.05).

K_m values are expressed in mmol 1⁻¹ADP, V_max values as nmol atom O min⁻¹mg⁻¹ mitochondrial protein.

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