Primer pairs used for ChIP assays
| Primer pair . | Sequence (5′ to 3′) . | |
|---|---|---|
| For ChIP assays of protein binding on the SOC1 sequence | ||
| 1 | GGATGCAACCTCCTTTCATGAG, ATATGGGTTTGGTTTCATTTGG | |
| 2 | AAAAACCTAACCAGGAGGAAGC, CTTCTTCTCCCTCCAGTAATGC | |
| 3 | GCAAAAGAAGTAGCTTTCCTCG, AGCAGAGAGAGAAGAGACGAGTG | |
| 4 | TGGACGCTTGAAACCTCATCCT, GGGAGGGAAAAAGATGTGTATG | |
| 5 | AGTTGGATGGAAATGCCTGTCA, TTACAAGTGGGGGCATATAGGT | |
| 6 | TCTCGTACCTATATGCCCCCACT, TTTATCTGTTGGGATGGAAAGA | |
| 7 | GAGGCTAGTACAGAGACAATGG, GACCAAAAATAGCAAATGCCTC | |
| 8 | TATATCGGGAGGAGGACCACAC, ATCCATACAGATTTTCGGACCT | |
| 9 | ATCACATCTCTTTGACGTTTGCTT, GCCCTAATTTTGCAGAAACCAA | |
| 10 | TGTTTCAGACATTTGGTCCATTTG, AGTCTTGTACTTTTTCCCCCTATTTTAG | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |
| For ChIP assays of protein binding on the AGL24 sequence | ||
| 1 | ACAAGTTCGAAATTTGGGCCA, TTCACGTTTTACCATTTGCCGT | |
| 2 | TGCTGTTCATCAGTTCATCTACC, CTTATCAGGTGTCGCATCTAG | |
| 3 | ATCCCCAATCATACCAAGTGAC, GTACTGGGAAATAAGAGAGCAG | |
| 4 | AGTTCAATCCATCAAGATCCTCTC, TCTTTGGTAGACCTACTGAACA | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |
| For ChIP assays of protein binding on the AP1 and LFYsequences | ||
| AP1-1 | CAAGTATCTTCTCCATACTGATC, TTATAAAGGTATCATAGAGATCGG | |
| AP1-2 | GAGTTAATTCTTTTTATGGATCCC, CATGTTTTGCAAATCTAAGCAAAG | |
| AP1-3 | GCAGTAGTGAATAATTAGGGCAA, AAGTTGCTCTTGTTGTCTTCTCCC | |
| AP1-4 | GTACGCAGGATTTAAGGAAAGAG, GATTTTTGTCCTGATCATCTACAAC | |
| AP1-5 | TTTGGTGTTTTCCACGTGTCTTC, ATAATACCGTAAGCAATAGTTGC | |
| LFY-1 | CTATACGACGTCGTTTGAAAGGGATCC, GCGTTTATATCTTCTCGGTCAGCCCA | |
| LFY-2 | TATCTTCCCCTAACAATACTTCCAAAGC, TCTTTGCAGAAGCCCGATAAGTTACT | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |
| Primer pair . | Sequence (5′ to 3′) . | |
|---|---|---|
| For ChIP assays of protein binding on the SOC1 sequence | ||
| 1 | GGATGCAACCTCCTTTCATGAG, ATATGGGTTTGGTTTCATTTGG | |
| 2 | AAAAACCTAACCAGGAGGAAGC, CTTCTTCTCCCTCCAGTAATGC | |
| 3 | GCAAAAGAAGTAGCTTTCCTCG, AGCAGAGAGAGAAGAGACGAGTG | |
| 4 | TGGACGCTTGAAACCTCATCCT, GGGAGGGAAAAAGATGTGTATG | |
| 5 | AGTTGGATGGAAATGCCTGTCA, TTACAAGTGGGGGCATATAGGT | |
| 6 | TCTCGTACCTATATGCCCCCACT, TTTATCTGTTGGGATGGAAAGA | |
| 7 | GAGGCTAGTACAGAGACAATGG, GACCAAAAATAGCAAATGCCTC | |
| 8 | TATATCGGGAGGAGGACCACAC, ATCCATACAGATTTTCGGACCT | |
| 9 | ATCACATCTCTTTGACGTTTGCTT, GCCCTAATTTTGCAGAAACCAA | |
| 10 | TGTTTCAGACATTTGGTCCATTTG, AGTCTTGTACTTTTTCCCCCTATTTTAG | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |
| For ChIP assays of protein binding on the AGL24 sequence | ||
| 1 | ACAAGTTCGAAATTTGGGCCA, TTCACGTTTTACCATTTGCCGT | |
| 2 | TGCTGTTCATCAGTTCATCTACC, CTTATCAGGTGTCGCATCTAG | |
| 3 | ATCCCCAATCATACCAAGTGAC, GTACTGGGAAATAAGAGAGCAG | |
| 4 | AGTTCAATCCATCAAGATCCTCTC, TCTTTGGTAGACCTACTGAACA | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |
| For ChIP assays of protein binding on the AP1 and LFYsequences | ||
| AP1-1 | CAAGTATCTTCTCCATACTGATC, TTATAAAGGTATCATAGAGATCGG | |
| AP1-2 | GAGTTAATTCTTTTTATGGATCCC, CATGTTTTGCAAATCTAAGCAAAG | |
| AP1-3 | GCAGTAGTGAATAATTAGGGCAA, AAGTTGCTCTTGTTGTCTTCTCCC | |
| AP1-4 | GTACGCAGGATTTAAGGAAAGAG, GATTTTTGTCCTGATCATCTACAAC | |
| AP1-5 | TTTGGTGTTTTCCACGTGTCTTC, ATAATACCGTAAGCAATAGTTGC | |
| LFY-1 | CTATACGACGTCGTTTGAAAGGGATCC, GCGTTTATATCTTCTCGGTCAGCCCA | |
| LFY-2 | TATCTTCCCCTAACAATACTTCCAAAGC, TCTTTGCAGAAGCCCGATAAGTTACT | |
| ACTIN | CGTTTCGCTTTCCTTAGTGTTAGCT, AGCGAACGGATCTAGAGACTCACCTTG | |