. | Rt (Ω cm2) . | . | |
---|---|---|---|
. | No TMAO . | TMAO . | |
48-h-explants | |||
Control | 22±3.9 (6) | 16±3.9 (6) | |
Heat stress | 32±5.1 (6) | 28±3.9 (6) | |
7.5-h-explants* | |||
Zinc-Free | 71±7.1 (5) | 72±13.1 (6) | |
50 μmol l–1 zinc | 62±11.1 (5) | 70±18.7 (6) | |
DMSO | KNK437 | ||
No TMAO-7.5-h-explants** | |||
Control | 37±5.0 (7) | 33±8.7 (7) | |
50 μmol l–1 zinc | 39±6.1 (8) | 25±5.6(8)*** |
. | Rt (Ω cm2) . | . | |
---|---|---|---|
. | No TMAO . | TMAO . | |
48-h-explants | |||
Control | 22±3.9 (6) | 16±3.9 (6) | |
Heat stress | 32±5.1 (6) | 28±3.9 (6) | |
7.5-h-explants* | |||
Zinc-Free | 71±7.1 (5) | 72±13.1 (6) | |
50 μmol l–1 zinc | 62±11.1 (5) | 70±18.7 (6) | |
DMSO | KNK437 | ||
No TMAO-7.5-h-explants** | |||
Control | 37±5.0 (7) | 33±8.7 (7) | |
50 μmol l–1 zinc | 39±6.1 (8) | 25±5.6(8)*** |
Values are means ± s.e.m. (N) following 90 min in Ussing Chambers. 7.5-h-Explants were assayed 7.5 h after removal from the animal;48-h-explants were maintained for 48–72 h in culture medium at 13.5°C. Heat stress was elevation of bath temperature +10°C to 23.5°C for 1 h followed by 1.5-h recovery at control temperature(13.5°C). Zinc exposure was 6 h followed by 1.5-h recovery in zinc-free medium
7.5-h-Explants had significantly higher Rt than 48-h-explants at P<0.05. Dimethyl sulfoxide (DMSO) was used at 0.2% as vehicle for 100 μmol l–1 KNK437
All tissues in DMSO had significantly lower Rt than 7.5-h-explants without DMSO at P<0.05
Rt was significantly lower in tissues treated with zinc plus KNK437 as compared to zinc plus DMSO at P<0.04