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Table 4.

Quantitative RT-PCR analysis of deps-1-regulated genes

A Genes upregulated in deps- 1 and rde- 3 mutants are not upregulated in rde-4 germ lines (isolated gonads, 20°C)     
  Amount of mRNA relative to wild-type gonads   
Wormbase ID endo-siRNAs* deps- 1(bn124) rde-4(ne301) Wild type§ 
C38D9.2 326.5 1.0 1.1 
F15D4.5 10 28.9 1.1 1.4 
T23G5.6 17.4 1.3 1.4 
Y55H10B.1 7.0 2.0 0.9 
C04F12.9 3.9 0.9 0.8 
B glh- 1 and deps- 1 are not downregulated in rde-3 or rde-4 mutants (gravid adults, 20°C)     
 Amount of mRNA in each mutant relative to wild type    
Wormbase ID (gene) deps- 1(bn124) rde-4(ne301) rde-3(ne298)  
T21G5.3 (glh-10.13 1.07 1.03  
Y65B4BL.2 (deps- 10.04 0.92 0.86  
T20G5.11 (rde-40.12, 0.14 0.20, 0.26 1.14  
C38D9.2 307.23 1.01 1330.44  
A Genes upregulated in deps- 1 and rde- 3 mutants are not upregulated in rde-4 germ lines (isolated gonads, 20°C)     
  Amount of mRNA relative to wild-type gonads   
Wormbase ID endo-siRNAs* deps- 1(bn124) rde-4(ne301) Wild type§ 
C38D9.2 326.5 1.0 1.1 
F15D4.5 10 28.9 1.1 1.4 
T23G5.6 17.4 1.3 1.4 
Y55H10B.1 7.0 2.0 0.9 
C04F12.9 3.9 0.9 0.8 
B glh- 1 and deps- 1 are not downregulated in rde-3 or rde-4 mutants (gravid adults, 20°C)     
 Amount of mRNA in each mutant relative to wild type    
Wormbase ID (gene) deps- 1(bn124) rde-4(ne301) rde-3(ne298)  
T21G5.3 (glh-10.13 1.07 1.03  
Y65B4BL.2 (deps- 10.04 0.92 0.86  
T20G5.11 (rde-40.12, 0.14 0.20, 0.26 1.14  
C38D9.2 307.23 1.01 1330.44  
*

Number of small anti-sense cDNAs isolated from each gene according to Table S1 in Lee et al. (Lee et al.,2006).

deps- 1 M-Z- mutants.

mRNA levels normalized relative to ama- 1 mRNA levels and compared with wild type.

§

Two independent wild-type samples were compared.

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