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Table 4.

Quantitative RT-PCR analysis of deps-1-regulated genes

A Genes upregulated in deps- 1 and rde- 3 mutants are not upregulated in rde-4 germ lines (isolated gonads, 20°C)

Amount of mRNA relative to wild-type gonads

Wormbase ID

endo-siRNAs^{^*}

deps- 1(bn124)^{^†}^{^‡}

rde-4(ne301)^{^‡}

Wild type^{^‡}^{^§}

C38D9.2

326.5

1.0

1.1

F15D4.5

28.9

1.1

1.4

T23G5.6

17.4

1.3

1.4

Y55H10B.1

7.0

2.0

0.9

C04F12.9

3.9

0.9

0.8

B glh- 1 and deps- 1 are not downregulated in rde-3 or rde-4 mutants (gravid adults, 20°C)

Amount of mRNA in each mutant relative to wild type

Wormbase ID (gene)

deps- 1(bn124)^{^†}^{^‡}

rde-4(ne301)^{^‡}

rde-3(ne298)^{^‡}

T21G5.3 (glh-1)

0.13

1.07

1.03

Y65B4BL.2 (deps- 1)

0.04

0.92

0.86

T20G5.11 (rde-4)

0.12, 0.14

0.20, 0.26

1.14

C38D9.2

307.23

1.01

1330.44

Number of small anti-sense cDNAs isolated from each gene according to Table S1 in Lee et al. (Lee et al.,2006).

†

deps- 1 M-Z- mutants.

‡

mRNA levels normalized relative to ama- 1 mRNA levels and compared with wild type.

Two independent wild-type samples were compared.

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