. | E10 . | . | . | E10.5 . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | WT . | Fgfr2Msx2-Cre . | Fgfr1/2Prx1-Cre . | WT . | Fgfr2Msx2-Cre . | Fgfr1/2Prx1-Cre . | ||||
pHH3 | ||||||||||
Forelimb | 7.1 (21) | 6.8 (15) | 6.9 (19) | 7.7 (20) | 5.8 (24) | 8.4 (13) | ||||
s.d. | 2.7 | 2.3 | 2.1 | 2.0 | 2.0 | 2.5 | ||||
P value | NS | NS | 0.003 | NS | ||||||
Hindlimb | 6.0 (16) | 4.6 (18) | 6.8 (5) | 4.0 (5) | ||||||
s.d. | 1.3 | 1.8 | 1.3 | 1.2 | ||||||
P value | 0.011 | 0.008 | ||||||||
Caspase 3 | ||||||||||
Forelimb | −/+ | −/+ | ++ | −/+ | + | −/+ | ||||
Hindlimb | −/+ | −/+ | −/+ | +++ |
. | E10 . | . | . | E10.5 . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | WT . | Fgfr2Msx2-Cre . | Fgfr1/2Prx1-Cre . | WT . | Fgfr2Msx2-Cre . | Fgfr1/2Prx1-Cre . | ||||
pHH3 | ||||||||||
Forelimb | 7.1 (21) | 6.8 (15) | 6.9 (19) | 7.7 (20) | 5.8 (24) | 8.4 (13) | ||||
s.d. | 2.7 | 2.3 | 2.1 | 2.0 | 2.0 | 2.5 | ||||
P value | NS | NS | 0.003 | NS | ||||||
Hindlimb | 6.0 (16) | 4.6 (18) | 6.8 (5) | 4.0 (5) | ||||||
s.d. | 1.3 | 1.8 | 1.3 | 1.2 | ||||||
P value | 0.011 | 0.008 | ||||||||
Caspase 3 | ||||||||||
Forelimb | −/+ | −/+ | ++ | −/+ | + | −/+ | ||||
Hindlimb | −/+ | −/+ | −/+ | +++ |
Shown are the average number of phosphohistone H3 (pHH3)-labeled cells in a 1.5×104 μm2 area adjacent to the AER, with the number of sections examined from at least three embryos in parentheses. Low frequency of caspase 3 (or TUNEL, not shown)-positive cells (−/+);transient proximal cell death (++); slightly increased distal mesenchymal cell death (+); cell death throughout the entire limb bud mesenchyme (+++).
NS, Not significantly different from wild type (WT).