The Company of Biologists

Table 1.

Summary of phenotypes in the NB3-1 lineage

	Protein gain or loss (+/−)	RP neuron identity^{^†}
Genotype^{^*}	Protein gain or loss (+/−)		Total # of RP	n	RP1	RP4	RP3	RP5	Conclusion
Wild type		4	100	1	1	1	1	Wild type
spdo mutant	− Sanpodo	8.0	70	2	2	2	2	Spdo specifies RP sibling fates
numb mutant	− Numb	1.0	88	1^{^‡}			0	0	Numb specifies RP fates
hb mutant	− Hb (CNS)	2	163	0	0	1	1	Hb is required for RP1/RP4 identity
2× UAS-hb	+ Hb	6.9	198	6.9^{^‡}			0	0	Hb is sufficient for RP1/4 identity
Kr mutant	− Kr (CNS)	2.7	152	1	1	0	0.7	Kr is required for RP3 identity
2× UAS-Kr	+ Kr	4.3	147	1	1	2.3	0	Kr is sufficient for RP3 identity
pdm mutant	− Pdm 1/2	5.1	172	1	1	1.8	1.3	Pdm closes the RP3 temporal window
2× UAS-pdm2	+ Pdm2	2.9	142	1	1	0.6	0.3	Pdm represses RP3/RP5 identity
cas mutant	− Cas	5.6	183	1	1	1	2.6	Castor closes the RP5 temporal window
2× UAS-cas	+ Cas	2.8	156	1	1	0.8	0	Cas represses RP5 identity

	Protein gain or loss (+/−)	RP neuron identity^{^†}
Genotype^{^*}	Protein gain or loss (+/−)		Total # of RP	n	RP1	RP4	RP3	RP5	Conclusion
Wild type		4	100	1	1	1	1	Wild type
spdo mutant	− Sanpodo	8.0	70	2	2	2	2	Spdo specifies RP sibling fates
numb mutant	− Numb	1.0	88	1^{^‡}			0	0	Numb specifies RP fates
hb mutant	− Hb (CNS)	2	163	0	0	1	1	Hb is required for RP1/RP4 identity
2× UAS-hb	+ Hb	6.9	198	6.9^{^‡}			0	0	Hb is sufficient for RP1/4 identity
Kr mutant	− Kr (CNS)	2.7	152	1	1	0	0.7	Kr is required for RP3 identity
2× UAS-Kr	+ Kr	4.3	147	1	1	2.3	0	Kr is sufficient for RP3 identity
pdm mutant	− Pdm 1/2	5.1	172	1	1	1.8	1.3	Pdm closes the RP3 temporal window
2× UAS-pdm2	+ Pdm2	2.9	142	1	1	0.6	0.3	Pdm represses RP3/RP5 identity
cas mutant	− Cas	5.6	183	1	1	1	2.6	Castor closes the RP5 temporal window
2× UAS-cas	+ Cas	2.8	156	1	1	0.8	0	Cas represses RP5 identity

P<0.01 for all experiments.

All genotypes are described in Materials and methods.

Average number of each cell type present per hemi-segment based on markers described in Fig. 1.

We currently lack the markers to distinguish between RP1 and RP4 in functional analyses.