The Company of Biologists

Table 3.

Left-right Ca_i²⁺ levels in chick embryos treated with serotonin agonists and antagonists, or proton ATPase antagonists

	Mean Fura-2 signal from line-scan analysis
Treatment	Right	Left	Ratio of left/right Ca_i²⁺ enrichment
Untreated	171.1±9.4 (3)	186.6±12.2	2.55±0.21 (3)
Fluoxetine, 10 μM	143.5±23.0 (11)^*	158.3±17.7^**	1.38±0.13 (6)^**
2-Methyl-5HT, 25 μM	135.0±21.8 (11)^**	153.6±17.6^**	0.52±0.26 (4)^**
Ondansetron, 25 μM	188.4±19.2 (10)^*	200.5±8.7^*	0.97±0.25 (6)^**
ML 10302, 25 μM	119.8±18.8 (9)^**	130.2±25.5^**	0.82±0.08 (6)^**
GR 125487, 25 μM	188.3±10.8 (10)^**	195.4±10.9	1.03±0.05 (10)^**
Concanamycin, 100 nM	151.2±13.7 (13)^**	150.8±13.6^**	1.04±0.24 (6)^**
Lansoprozole, 7 μM	186.1±13.7 (14)^**	183.8±18.1	1.03±0.19 (7)^**

	Mean Fura-2 signal from line-scan analysis
Treatment	Right	Left	Ratio of left/right Ca_i²⁺ enrichment
Untreated	171.1±9.4 (3)	186.6±12.2	2.55±0.21 (3)
Fluoxetine, 10 μM	143.5±23.0 (11)^*	158.3±17.7^**	1.38±0.13 (6)^**
2-Methyl-5HT, 25 μM	135.0±21.8 (11)^**	153.6±17.6^**	0.52±0.26 (4)^**
Ondansetron, 25 μM	188.4±19.2 (10)^*	200.5±8.7^*	0.97±0.25 (6)^**
ML 10302, 25 μM	119.8±18.8 (9)^**	130.2±25.5^**	0.82±0.08 (6)^**
GR 125487, 25 μM	188.3±10.8 (10)^**	195.4±10.9	1.03±0.05 (10)^**
Concanamycin, 100 nM	151.2±13.7 (13)^**	150.8±13.6^**	1.04±0.24 (6)^**
Lansoprozole, 7 μM	186.1±13.7 (14)^**	183.8±18.1	1.03±0.19 (7)^**

Left and right mean Fura-2 signal, relative Ca_i²⁺levels and the LR Ca_i²⁺ ratio were calculated as described in the Materials and methods. The number of embryos analyzed is indicated in parentheses. Asterisks indicate that values differ from HH6 controls on that side at ^*P<0.05 and ^**P<0.005 using an unpaired t-test.

View Large

This Feature Is Available To Subscribers Only