Neurons forming the trigeminal sensory ganglia
| Conditions . | Stage . | huc:kaede . | p2x3b:egfp . | trpa1b:egfp . |
|---|---|---|---|---|
| Wild type | 24 hpf | 37±3 (n=5) | 15±2 (n=11) | 20±3 (n=5) |
| 72 hpf | 53±6 (n=5) | 17±2 (n=7) | 13±2 (n=7) | |
| Early-born neurons: 35±4 | Early-born neurons: 14±1 | Early-born neurons: 13±2 | ||
| Late-born neurons: 18±3 | Late-born neurons: 3±1 | Late-born neurons: 0 | ||
| +Anti-proliferative drugs | 72 hpf | 34±3 (n=11) | 17±3 (n=5) | 20±1 (n=5) |
| Early-born neurons: 32±3 | Mock treated: 18±1 (n=3) | Mock treated: 24±3 (n=3) | ||
| Late-born neurons: 3±1 | ||||
| +neurogenin1 morpholino | 72 hpf | 15±2 (n=8) | 8±2 (n=3) | 0 (n=11) |
| Conditions . | Stage . | huc:kaede . | p2x3b:egfp . | trpa1b:egfp . |
|---|---|---|---|---|
| Wild type | 24 hpf | 37±3 (n=5) | 15±2 (n=11) | 20±3 (n=5) |
| 72 hpf | 53±6 (n=5) | 17±2 (n=7) | 13±2 (n=7) | |
| Early-born neurons: 35±4 | Early-born neurons: 14±1 | Early-born neurons: 13±2 | ||
| Late-born neurons: 18±3 | Late-born neurons: 3±1 | Late-born neurons: 0 | ||
| +Anti-proliferative drugs | 72 hpf | 34±3 (n=11) | 17±3 (n=5) | 20±1 (n=5) |
| Early-born neurons: 32±3 | Mock treated: 18±1 (n=3) | Mock treated: 24±3 (n=3) | ||
| Late-born neurons: 3±1 | ||||
| +neurogenin1 morpholino | 72 hpf | 15±2 (n=8) | 8±2 (n=3) | 0 (n=11) |
Neurons expressing huc:kaede, p2×3b:egfpand/or trpa1b:egfp transgenes were counted in 24 hpf and 72 hpf embryos. The BAPTISM technique was used to distinguish early-born neurons from late-born neurons at 72 hpf. The averages reported in the table represent the number of neurons per ganglion expressing a particular transgene in wild-type embryos, embryos treated with anti-proliferation drugs or neurogenin1 morphant embryos. The standard error and sample size are indicated.