Microinjection of antibodies against phosphorylated serine 353 delays entry into mitosis
| A | |||
| Exp. no. | Antibodies | Time after microinjection (hours) | Divided cells* |
| 1 | IgG | 7 | 26% |
| SE96 | 7 | 19% | |
| 2 | IgG | 20 | 53% |
| SE96 | 20 | 27% | |
| 3 | IgG | 21 | 61% |
| SE96 | 21 | 45% | |
| B | |||
| Antibodies | Time of microinjection (hours) | Cell number | Divided cells* |
| IgG | 10 | 103 | 32% |
| SE96 | 9 | 81 | 10% |
| SE96 | 10.5 | 64 | 18% |
| A | |||
| Exp. no. | Antibodies | Time after microinjection (hours) | Divided cells* |
| 1 | IgG | 7 | 26% |
| SE96 | 7 | 19% | |
| 2 | IgG | 20 | 53% |
| SE96 | 20 | 27% | |
| 3 | IgG | 21 | 61% |
| SE96 | 21 | 45% | |
| B | |||
| Antibodies | Time of microinjection (hours) | Cell number | Divided cells* |
| IgG | 10 | 103 | 32% |
| SE96 | 9 | 81 | 10% |
| SE96 | 10.5 | 64 | 18% |
Exp. no., experiment number.
Affinity-purified anti-CDC25B antibodies (SE96) or normal rabbit IgG were injected into the nucleus of scattered HeLa cells (as described by Lane and Nigg, 1996) either asynchronous or synchronised by thymidine block. (A) Asynchronous HeLa cells fixed at the indicated time after microinjection. (B) HeLa cells synchronised by thymidine block, injected in G2 at the indicated time after release, and fixed 16 hours after block release.
The percentage of divided cells among the microinjected cells population (detected by staining with anti-rabbit IgG antibody as two fluorescent cells next to each other).