Percentage of animals displaying a punctate (nuclear) DAF-16::GFP pattern at 20°C
| Genotype . | % Punctate GFP±s.d.(n)* . | Development time(stage)† . | % Punctate GFP dauers(n)‡ . |
|---|---|---|---|
| Cholesterol | |||
| hsd-1(mg433); ncr-1(nr2022); zls356 | 29.0±12.8 (177) | 46.5-48.5 hours (L2d) | 3.1 (97) |
| ncr-2(2023); ncr-1(nr2022); zls356 | 60.6±5.3 (264) | 46.5-48.5 hours (L2d) | 0 (160) |
| zls356 | 0±0 (207) | 38.5-40.5 hours (L2) | - |
| zls356 | 0±0 (224) | 44.5 hours (L2/L3) | - |
| zls356 | 0±0 (142) | 45.5 hours (L2/L3) | - |
| 4-cholesten-3-one | |||
| hsd-1(mg433); ncr-1(nr2022); zls356 | 0±0 (226) | 38.5-40.5 hours (L2) | - |
| hsd-1(mg433); ncr-1(nr2022); zls356 | 1.4±0.2 (135) | 46.5-48.5 hours (L2/L3) | - |
| ncr-2(2023); ncr-1(nr2022); zls356 | 0±0 (87) | 38.5-40.5 hours (L2) | - |
| ncr-2(2023); ncr-1(nr2022); zls356 | 0±0 (136) | 46.5-48.5 hours (L2/L3) | - |
| zls356 | 0±0 (87) | 38.5-40.5 hours (L2) | - |
| zls356 | 0±0 (108) | 46.5-48.5 hrs (L2/L3) | - |
| Genotype . | % Punctate GFP±s.d.(n)* . | Development time(stage)† . | % Punctate GFP dauers(n)‡ . |
|---|---|---|---|
| Cholesterol | |||
| hsd-1(mg433); ncr-1(nr2022); zls356 | 29.0±12.8 (177) | 46.5-48.5 hours (L2d) | 3.1 (97) |
| ncr-2(2023); ncr-1(nr2022); zls356 | 60.6±5.3 (264) | 46.5-48.5 hours (L2d) | 0 (160) |
| zls356 | 0±0 (207) | 38.5-40.5 hours (L2) | - |
| zls356 | 0±0 (224) | 44.5 hours (L2/L3) | - |
| zls356 | 0±0 (142) | 45.5 hours (L2/L3) | - |
| 4-cholesten-3-one | |||
| hsd-1(mg433); ncr-1(nr2022); zls356 | 0±0 (226) | 38.5-40.5 hours (L2) | - |
| hsd-1(mg433); ncr-1(nr2022); zls356 | 1.4±0.2 (135) | 46.5-48.5 hours (L2/L3) | - |
| ncr-2(2023); ncr-1(nr2022); zls356 | 0±0 (87) | 38.5-40.5 hours (L2) | - |
| ncr-2(2023); ncr-1(nr2022); zls356 | 0±0 (136) | 46.5-48.5 hours (L2/L3) | - |
| zls356 | 0±0 (87) | 38.5-40.5 hours (L2) | - |
| zls356 | 0±0 (108) | 46.5-48.5 hrs (L2/L3) | - |
Most animals have a mixed pattern of punctate and diffuse GFP. In this data set, only 2.7% (n=177) of hsd-1; ncr-1 and 0%(n=264) ncr-2; ncr-1 displayed a complete punctate pattern.
Time elapsed between the mid-point of the egg-lay and scoring. Approximate developmental stages are indicated in brackets.
Scored 70 hours after egg-laying, while animals are still in the dauer stage.