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Table 3.

Percentage of animals displaying a punctate (nuclear) DAF-16::GFP pattern at 20°C

Genotype% Punctate GFP±s.d.(n)*Development time(stage)% Punctate GFP dauers(n)
Cholesterol
 

 

 

 
hsd-1(mg433); ncr-1(nr2022); zls356 29.0±12.8 (177) 46.5-48.5 hours (L2d) 3.1 (97) 
ncr-2(2023); ncr-1(nr2022); zls356 60.6±5.3 (264) 46.5-48.5 hours (L2d) 0 (160) 
zls356 0±0 (207) 38.5-40.5 hours (L2) 
zls356 0±0 (224) 44.5 hours (L2/L3) 
zls356
 
0±0 (142)
 
45.5 hours (L2/L3)
 
-
 
4-cholesten-3-one
 

 

 

 
hsd-1(mg433); ncr-1(nr2022); zls356 0±0 (226) 38.5-40.5 hours (L2) 
hsd-1(mg433); ncr-1(nr2022); zls356 1.4±0.2 (135) 46.5-48.5 hours (L2/L3) 
ncr-2(2023); ncr-1(nr2022); zls356 0±0 (87) 38.5-40.5 hours (L2) 
ncr-2(2023); ncr-1(nr2022); zls356 0±0 (136) 46.5-48.5 hours (L2/L3) 
zls356 0±0 (87) 38.5-40.5 hours (L2) 
zls356 0±0 (108) 46.5-48.5 hrs (L2/L3) 
Genotype% Punctate GFP±s.d.(n)*Development time(stage)% Punctate GFP dauers(n)
Cholesterol
 

 

 

 
hsd-1(mg433); ncr-1(nr2022); zls356 29.0±12.8 (177) 46.5-48.5 hours (L2d) 3.1 (97) 
ncr-2(2023); ncr-1(nr2022); zls356 60.6±5.3 (264) 46.5-48.5 hours (L2d) 0 (160) 
zls356 0±0 (207) 38.5-40.5 hours (L2) 
zls356 0±0 (224) 44.5 hours (L2/L3) 
zls356
 
0±0 (142)
 
45.5 hours (L2/L3)
 
-
 
4-cholesten-3-one
 

 

 

 
hsd-1(mg433); ncr-1(nr2022); zls356 0±0 (226) 38.5-40.5 hours (L2) 
hsd-1(mg433); ncr-1(nr2022); zls356 1.4±0.2 (135) 46.5-48.5 hours (L2/L3) 
ncr-2(2023); ncr-1(nr2022); zls356 0±0 (87) 38.5-40.5 hours (L2) 
ncr-2(2023); ncr-1(nr2022); zls356 0±0 (136) 46.5-48.5 hours (L2/L3) 
zls356 0±0 (87) 38.5-40.5 hours (L2) 
zls356 0±0 (108) 46.5-48.5 hrs (L2/L3) 
*

Most animals have a mixed pattern of punctate and diffuse GFP. In this data set, only 2.7% (n=177) of hsd-1; ncr-1 and 0%(n=264) ncr-2; ncr-1 displayed a complete punctate pattern.

Time elapsed between the mid-point of the egg-lay and scoring. Approximate developmental stages are indicated in brackets.

Scored 70 hours after egg-laying, while animals are still in the dauer stage.

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