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Table 2.

Rescue of hsd-1(mg433); ncr-1(nr2022) dauer arrest with extrachromosomal arrays (20°C, 68 hours post egg-lay)

% Transgenic adults [mean±s.d. (n)]
Transgene*Line 1Line 2Line 3
No transgene control 0±0 (177) 
Psdf-9::hsd-1 99.5±0.8 (413) 98.0±1.7 (604) 95.8±4.0 (666) 
Phsd-1::hsd-1 95.2±3.7 (202) 
% Transgenic adults [mean±s.d. (n)]
Transgene*Line 1Line 2Line 3
No transgene control 0±0 (177) 
Psdf-9::hsd-1 99.5±0.8 (413) 98.0±1.7 (604) 95.8±4.0 (666) 
Phsd-1::hsd-1 95.2±3.7 (202) 
*

Both transgenes were co-injected with SUR-5::GFP. Animals were scored as`transgenic' based on visualization of GFP. We found that worms scored as`non-transgenic' siblings had low levels of dauer rescue, unlike the no transgene control. Phsd-1::hsd-1 `non-transgenic' siblings formed 7.1% (n=463), 12.7% (n=371) and 4.2% (n=551) adults in lines 1, 2 and 3, respectively. Phsd-1::hsd-1 `non-transgenic'siblings formed 0.6% adults (n=126). One possibility is that the adult `non-transgenic' siblings carry transgenes at very low copy numbers that cannot be visualized by GFP.

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