Dauer arrest at different temperatures . | . | . | . | ||
---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | ||
Genotype . | 25°C . | 20°C . | 15°C . | ||
hsd-1(mg433); ncr-1(nr2022) | 98.3±0.9 (844) | 99.9±0.2 (487) | 99.4±0.4 (1025) | ||
hsd-1(mg433) | 0±0 (864) | - | - | ||
ncr-1(nr2022) | 0±0 (317) | 0±0 (399) | 0±0 (218) | ||
hsd-1(mg433); ncr-1(nr2022); daf-12(RNAi) | 1.9±1.3 (807) | - | - | ||
hsd-1(mg433); ncr-2(nr2023) | 0±0 (449) | 0±0 (247) | - | ||
hsd-1(mg433);daf-36(k114)* | 100±0 (476) | - | - | ||
daf-36(k114) | 2.3±2.8 (925) | - | - | ||
hsd-1(mg433); daf-28(tm2308) | 89.6±5.2 (1190) | 79.8±10.4 (336) | 88.2±5.6 (274) | ||
daf-28(tm2308) | 28.5±5.3 (2475) | 0±0 (584) | 0±0 (328) |
Dauer arrest at different temperatures . | . | . | . | ||
---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | ||
Genotype . | 25°C . | 20°C . | 15°C . | ||
hsd-1(mg433); ncr-1(nr2022) | 98.3±0.9 (844) | 99.9±0.2 (487) | 99.4±0.4 (1025) | ||
hsd-1(mg433) | 0±0 (864) | - | - | ||
ncr-1(nr2022) | 0±0 (317) | 0±0 (399) | 0±0 (218) | ||
hsd-1(mg433); ncr-1(nr2022); daf-12(RNAi) | 1.9±1.3 (807) | - | - | ||
hsd-1(mg433); ncr-2(nr2023) | 0±0 (449) | 0±0 (247) | - | ||
hsd-1(mg433);daf-36(k114)* | 100±0 (476) | - | - | ||
daf-36(k114) | 2.3±2.8 (925) | - | - | ||
hsd-1(mg433); daf-28(tm2308) | 89.6±5.2 (1190) | 79.8±10.4 (336) | 88.2±5.6 (274) | ||
daf-28(tm2308) | 28.5±5.3 (2475) | 0±0 (584) | 0±0 (328) |
Dauer arrest induced by pheromone† . | . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | . | . | . | |||||
Genotype . | 0.7 μl . | 1.4 μl . | 2.75 μl . | 5.5 μl . | 11.0 μl . | 22.0 μl . | |||||
N2 | - | - | 1.6% | 2.8% | 19.1% | 50.5% | |||||
hsd-1(mg433) | 50.7% | 94.9% | 98.9% | 99.6% | 99.7% | 100% |
Dauer arrest induced by pheromone† . | . | . | . | . | . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | . | . | . | |||||
Genotype . | 0.7 μl . | 1.4 μl . | 2.75 μl . | 5.5 μl . | 11.0 μl . | 22.0 μl . | |||||
N2 | - | - | 1.6% | 2.8% | 19.1% | 50.5% | |||||
hsd-1(mg433) | 50.7% | 94.9% | 98.9% | 99.6% | 99.7% | 100% |
Dauer recovery‡ . | . | . | . | . | |||
---|---|---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | . | |||
Genotype . | Day 1 . | Day 2 . | Day 3 . | Day 4 . | |||
hsd-1(mg433); ncr-1(nr2022) | 12% | 64% | 97.8% | 100% | |||
ncr-2(nr2023); ncr-1(nr2022) | 40% | 88% | 100% | - |
Dauer recovery‡ . | . | . | . | . | |||
---|---|---|---|---|---|---|---|
. | Mean dauer arrest±s.d. (n) . | . | . | . | |||
Genotype . | Day 1 . | Day 2 . | Day 3 . | Day 4 . | |||
hsd-1(mg433); ncr-1(nr2022) | 12% | 64% | 97.8% | 100% | |||
ncr-2(nr2023); ncr-1(nr2022) | 40% | 88% | 100% | - |
Synchronized populations of this strain were obtained using the egg/embryo preparation method (see Materials and methods). For all other strains,populations were synchronized using timed egg-lays.
This batch of pheromone was different to that used in Fig. 5B.
Recovery tests were done at 25°C. Dauers were picked 72 hours post egg-lay (day 0) and then monitored daily for recovery to adults. Percentages shown represent cumulative recovery.