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Table 1.

Percentage of animals that undergo dauer arrest in different conditions and recovery times for double mutants

Dauer arrest at different temperatures



Mean dauer arrest±s.d. (n)
Genotype25°C20°C15°C
hsd-1(mg433); ncr-1(nr2022) 98.3±0.9 (844) 99.9±0.2 (487) 99.4±0.4 (1025) 
hsd-1(mg433) 0±0 (864) 
ncr-1(nr2022) 0±0 (317) 0±0 (399) 0±0 (218) 
hsd-1(mg433); ncr-1(nr2022); daf-12(RNAi) 1.9±1.3 (807) 
hsd-1(mg433); ncr-2(nr2023) 0±0 (449) 0±0 (247) 
hsd-1(mg433);daf-36(k114)* 100±0 (476) 
daf-36(k114) 2.3±2.8 (925) 
hsd-1(mg433); daf-28(tm2308) 89.6±5.2 (1190) 79.8±10.4 (336) 88.2±5.6 (274) 
daf-28(tm2308) 28.5±5.3 (2475) 0±0 (584) 0±0 (328) 
Dauer arrest at different temperatures



Mean dauer arrest±s.d. (n)
Genotype25°C20°C15°C
hsd-1(mg433); ncr-1(nr2022) 98.3±0.9 (844) 99.9±0.2 (487) 99.4±0.4 (1025) 
hsd-1(mg433) 0±0 (864) 
ncr-1(nr2022) 0±0 (317) 0±0 (399) 0±0 (218) 
hsd-1(mg433); ncr-1(nr2022); daf-12(RNAi) 1.9±1.3 (807) 
hsd-1(mg433); ncr-2(nr2023) 0±0 (449) 0±0 (247) 
hsd-1(mg433);daf-36(k114)* 100±0 (476) 
daf-36(k114) 2.3±2.8 (925) 
hsd-1(mg433); daf-28(tm2308) 89.6±5.2 (1190) 79.8±10.4 (336) 88.2±5.6 (274) 
daf-28(tm2308) 28.5±5.3 (2475) 0±0 (584) 0±0 (328) 
Dauer arrest induced by pheromone





Mean dauer arrest±s.d. (n)
Genotype0.7 μl1.4 μl2.75 μl5.5 μl11.0 μl22.0 μl
N2 1.6% 2.8% 19.1% 50.5% 
hsd-1(mg433) 50.7% 94.9% 98.9% 99.6% 99.7% 100% 
Dauer arrest induced by pheromone





Mean dauer arrest±s.d. (n)
Genotype0.7 μl1.4 μl2.75 μl5.5 μl11.0 μl22.0 μl
N2 1.6% 2.8% 19.1% 50.5% 
hsd-1(mg433) 50.7% 94.9% 98.9% 99.6% 99.7% 100% 
Dauer recovery




Mean dauer arrest±s.d. (n)
GenotypeDay 1Day 2Day 3Day 4
hsd-1(mg433); ncr-1(nr2022) 12% 64% 97.8% 100% 
ncr-2(nr2023); ncr-1(nr2022) 40% 88% 100% 
Dauer recovery




Mean dauer arrest±s.d. (n)
GenotypeDay 1Day 2Day 3Day 4
hsd-1(mg433); ncr-1(nr2022) 12% 64% 97.8% 100% 
ncr-2(nr2023); ncr-1(nr2022) 40% 88% 100% 
*

Synchronized populations of this strain were obtained using the egg/embryo preparation method (see Materials and methods). For all other strains,populations were synchronized using timed egg-lays.

This batch of pheromone was different to that used in Fig. 5B.

Recovery tests were done at 25°C. Dauers were picked 72 hours post egg-lay (day 0) and then monitored daily for recovery to adults. Percentages shown represent cumulative recovery.

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