Antibodies . | Source . | Dilution used . |
---|---|---|
Primary | ||
Mouse anti-APC, clone CC1 | Calbiochem | 1:200 |
Mouse anti-CD31 | BD Sciences | 1:500 |
Rabbit anti-Cre | Novagen | 1:5000 |
Rat anti-F4/80 | Serotec | 1:200 |
Rabbit anti-β-galactosidase | Eppendorf Five Prime | 1:500 |
Mouse anti-GFAP | Sigma | 1:1000 |
Rabbit anti-GFAP | DakoCytomation | 1:1000 |
Guinea pig anti-GLAST | Chemicon | 1:1000 |
Mouse anti-GFP | Chemicon | 1:500 |
Rabbit anti-GFP | Chemicon | 1:1000 |
Mouse anti-myelin basic protein (Smi99) | Sternberger Monoclonals | 1:5000 |
Mouse anti-nestin | Chemicon | 1:50-1:200 |
Mouse anti-NeuN | Chemicon | 1:200 |
Rabbit anti-rat NG2 | Dr W. Stallcup | 1:1000 |
Dr J. Levine | 1:1000 | |
Chemicon | 1:500 | |
Guinea pig anti-rat NG2 | Dr W. Stallcup | 1:1000 |
Rat anti-mouse NG2 | Dr J. Trotter | 1:1000 |
Mouse anti-O4 and O1 | Dr S. Pfeiffer | 1:5 (supernatant) |
Rabbit anti-PDGFRα | Dr W. Stallcup | 1:1000 |
Rabbit anti-PDGFRβ | Dr W. Stallcup | 1:1000 |
Mouse anti-S100β | Sigma | 1:2000 |
Mouse anti-βIII-tubulin | Chemicon | 1:200 |
Secondary | ||
FITC-, Cy3- and Cy5-conjugated anti-mouse, anti-rabbit, anti-rat and anti-guinea pig antibodies | Jackson ImmunoResearch | FITC 1:50 Cy3 1:200 Cy5 1:50 |
Alexa 488-conjugated anti-mouse or rabbit | Molecular Probes | 1:2000 |
Antibodies . | Source . | Dilution used . |
---|---|---|
Primary | ||
Mouse anti-APC, clone CC1 | Calbiochem | 1:200 |
Mouse anti-CD31 | BD Sciences | 1:500 |
Rabbit anti-Cre | Novagen | 1:5000 |
Rat anti-F4/80 | Serotec | 1:200 |
Rabbit anti-β-galactosidase | Eppendorf Five Prime | 1:500 |
Mouse anti-GFAP | Sigma | 1:1000 |
Rabbit anti-GFAP | DakoCytomation | 1:1000 |
Guinea pig anti-GLAST | Chemicon | 1:1000 |
Mouse anti-GFP | Chemicon | 1:500 |
Rabbit anti-GFP | Chemicon | 1:1000 |
Mouse anti-myelin basic protein (Smi99) | Sternberger Monoclonals | 1:5000 |
Mouse anti-nestin | Chemicon | 1:50-1:200 |
Mouse anti-NeuN | Chemicon | 1:200 |
Rabbit anti-rat NG2 | Dr W. Stallcup | 1:1000 |
Dr J. Levine | 1:1000 | |
Chemicon | 1:500 | |
Guinea pig anti-rat NG2 | Dr W. Stallcup | 1:1000 |
Rat anti-mouse NG2 | Dr J. Trotter | 1:1000 |
Mouse anti-O4 and O1 | Dr S. Pfeiffer | 1:5 (supernatant) |
Rabbit anti-PDGFRα | Dr W. Stallcup | 1:1000 |
Rabbit anti-PDGFRβ | Dr W. Stallcup | 1:1000 |
Mouse anti-S100β | Sigma | 1:2000 |
Mouse anti-βIII-tubulin | Chemicon | 1:200 |
Secondary | ||
FITC-, Cy3- and Cy5-conjugated anti-mouse, anti-rabbit, anti-rat and anti-guinea pig antibodies | Jackson ImmunoResearch | FITC 1:50 Cy3 1:200 Cy5 1:50 |
Alexa 488-conjugated anti-mouse or rabbit | Molecular Probes | 1:2000 |
Materials were obtained from Dr W. Stallcup (La Jolla, CA), Dr J. Levine(Stony Brook, NY), Dr J. Trotter (Mainz, Germany) and Dr S. Pfeiffer(Farmington, CT).