Cell cycle timing* . | . | . | . | . |
---|---|---|---|---|
Cell cycle . | Wild type (6) . | mnk (chk2) (5) . | grp (chk1) (5) . | mnk grp (9) . |
10 I | 4.7±0.43 | 4.7±0.48 | 4.7±0.44 | 4.9±0.51 |
M | 4.4±0.90 | 4.0±0.54 | 4.5±0.75 | 4.8±0.60 |
11 I | 5.2±0.50 | 4.4±0.49 | 4.9±0.75 | 5.3±0.67 |
M | 4.3±1.00 | 4.0±0.49 | 3.9±0.25 | 4.5±0.47 |
12 I | 7.2±0.85 | 8.4±1.00 | 6.1±0.16 | 6.5±0.69 |
M | 4.6±0.41 | 4.3±0.54 | 4.8±0.89 | 5.0±0.37 |
13 I | 13.9±1.42 | 15.9±2.24 | 7.1±0.83 | 9.7±0.93 |
M | 4.8±0.54 | 5.0±0.83 | 8.1±0.80 | 4.9±0.71 |
14 I | NA | NA | 10.9±0.62 | 12.6±1.20 |
M | NA | NA | 13.8±1.38 | 6.7±0.89 |
Cell cycle timing* . | . | . | . | . |
---|---|---|---|---|
Cell cycle . | Wild type (6) . | mnk (chk2) (5) . | grp (chk1) (5) . | mnk grp (9) . |
10 I | 4.7±0.43 | 4.7±0.48 | 4.7±0.44 | 4.9±0.51 |
M | 4.4±0.90 | 4.0±0.54 | 4.5±0.75 | 4.8±0.60 |
11 I | 5.2±0.50 | 4.4±0.49 | 4.9±0.75 | 5.3±0.67 |
M | 4.3±1.00 | 4.0±0.49 | 3.9±0.25 | 4.5±0.47 |
12 I | 7.2±0.85 | 8.4±1.00 | 6.1±0.16 | 6.5±0.69 |
M | 4.6±0.41 | 4.3±0.54 | 4.8±0.89 | 5.0±0.37 |
13 I | 13.9±1.42 | 15.9±2.24 | 7.1±0.83 | 9.7±0.93 |
M | 4.8±0.54 | 5.0±0.83 | 8.1±0.80 | 4.9±0.71 |
14 I | NA | NA | 10.9±0.62 | 12.6±1.20 |
M | NA | NA | 13.8±1.38 | 6.7±0.89 |
. | Wild type . | mnk (chk2) . | grp (chk1) . | mnk grp . |
---|---|---|---|---|
Int | 71.0±2.89 (6) | 68.9±2.42 (5) | NA | 83.5±3.15 (8) |
Mid | 104.6±5.15 (6) | 102.3±4.05 (5) | NA | 125.7±6.10 (6) |
. | Wild type . | mnk (chk2) . | grp (chk1) . | mnk grp . |
---|---|---|---|---|
Int | 71.0±2.89 (6) | 68.9±2.42 (5) | NA | 83.5±3.15 (8) |
Mid | 104.6±5.15 (6) | 102.3±4.05 (5) | NA | 125.7±6.10 (6) |
Pole-cell migration to cellularization**
Average durations±s.d. of cell cycle timing are shown. Timing of nuclear envelope formation (NEF) and nuclear envelope breakdown (NEB) were measured from DIC live recordings of embryos. I, interphase from NEF to NEB;M, mitosis from NEB to NEF. Number of embryos analyzed are shown in parentheses.
Average durations±s.d. from pole-cell migration (pole-bud formation)to cellularization are shown. Number of embryos analyzed are shown in parentheses. Int, initiation of membrane invagination; Mid, invaginating membrane reaches to approximately one third of the final cell length; NA, not analyzed.