. | Viscera . | . | . | . | . | Pronephric . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
[pkd2 augMO] . | n . | Situs solitus (%) . | Situs inversus (%) . | Heterotaxia (%) . | Abnormal (%) . | n . | Dilation (%) . | |||||
Control | 45 | 100.0 | 0.0 | 0.0 | 0.0 | 29 | 0.0 | |||||
1 ng | 66 | 63.6 | 27.3 | 9.1 | 0.0 | 38 | 5.3 | |||||
2 ng | 54 | 53.7 | 35.2 | 11.1 | 0.0 | 44 | 65.9 | |||||
3 ng | 84 | 25.0 | 42.9 | 6.0 | 26.1 | 53 | 60.4 | |||||
4 ng | 56 | 0.0 | 0.0 | 0.0 | 100.0 | 46 | 97.8 |
. | Viscera . | . | . | . | . | Pronephric . | . | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
[pkd2 augMO] . | n . | Situs solitus (%) . | Situs inversus (%) . | Heterotaxia (%) . | Abnormal (%) . | n . | Dilation (%) . | |||||
Control | 45 | 100.0 | 0.0 | 0.0 | 0.0 | 29 | 0.0 | |||||
1 ng | 66 | 63.6 | 27.3 | 9.1 | 0.0 | 38 | 5.3 | |||||
2 ng | 54 | 53.7 | 35.2 | 11.1 | 0.0 | 44 | 65.9 | |||||
3 ng | 84 | 25.0 | 42.9 | 6.0 | 26.1 | 53 | 60.4 | |||||
4 ng | 56 | 0.0 | 0.0 | 0.0 | 100.0 | 46 | 97.8 |
Injections were performed between the one- and four-cell stage in a 5 mg/ml Phenol Red solution. Control injections were with Phenol Red solution alone. The abnormal viscera category includes endoderm defects ranging from multiple liver and pancreata, to thinner body axes that only allowed for midline and improperly shaped abdominal organs. At 4 days, the injected embryos had what appeared to be cystic pronephric regions that differed in size and shape as compared with our normal definition of a kidney cyst. Therefore, we refer to this phenotype as a kidney dilation. Embryos injected with 2 ng or more of the augMO had upward curvatures in their body axes.