Alterations in asymmetric gene expression in cup mutant embryos
| . | . | spaw . | . | . | . | . | pitx2 . | . | . | . | . | lefty1/2 . | . | . | . | . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tissue . | Genotype . | n . | L(%) . | B(%) . | R(%) . | A(%) . | n . | L(%) . | B(%) . | R(%) . | A(%) . | n . | L(%) . | B(%) . | R(%) . | A(%) . | ||||||||||||
| LPM | Wild type | 90 | 93.3 | 5.6 | 0.0 | 1.1 | 116 | 95.7 | 3.4 | 0.9 | 0.0 | 27 | 100 | 0.0 | 0.0 | 0.0 | ||||||||||||
| cuptc321 | 114 | 73.7 | 20.2 | 6.1 | 0.0 | 192 | 63.6 | 16.2 | 16.6 | 3.6 | 114 | 69.3 | 1.8 | 3.5 | 25.4 | |||||||||||||
| cupty30b | 102 | 57.8 | 29.5 | 2.9 | 9.8 | 168 | 64.9 | 15.5 | 19.6 | 0.0 | 95 | 57.9 | 3.1 | 7.4 | 31.6 | |||||||||||||
| Brain | Wild type | - | - | - | - | - | 116 | 94.8 | 1.7 | 0.9 | 2.6 | 27 | 100 | 0.0 | 0.0 | 0.0 | ||||||||||||
| cuptc321 | - | - | - | - | - | 101 | 55.4 | 3.0 | 4.0 | 37.6 | 114 | 67.6 | 1.7 | 1.7 | 29.0 | |||||||||||||
| cupty30b | - | - | - | - | - | 169 | 58.0 | 5.3 | 9.5 | 27.2 | 95 | 50.5 | 2.1 | 4.2 | 43.2 | |||||||||||||
| . | . | spaw . | . | . | . | . | pitx2 . | . | . | . | . | lefty1/2 . | . | . | . | . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tissue . | Genotype . | n . | L(%) . | B(%) . | R(%) . | A(%) . | n . | L(%) . | B(%) . | R(%) . | A(%) . | n . | L(%) . | B(%) . | R(%) . | A(%) . | ||||||||||||
| LPM | Wild type | 90 | 93.3 | 5.6 | 0.0 | 1.1 | 116 | 95.7 | 3.4 | 0.9 | 0.0 | 27 | 100 | 0.0 | 0.0 | 0.0 | ||||||||||||
| cuptc321 | 114 | 73.7 | 20.2 | 6.1 | 0.0 | 192 | 63.6 | 16.2 | 16.6 | 3.6 | 114 | 69.3 | 1.8 | 3.5 | 25.4 | |||||||||||||
| cupty30b | 102 | 57.8 | 29.5 | 2.9 | 9.8 | 168 | 64.9 | 15.5 | 19.6 | 0.0 | 95 | 57.9 | 3.1 | 7.4 | 31.6 | |||||||||||||
| Brain | Wild type | - | - | - | - | - | 116 | 94.8 | 1.7 | 0.9 | 2.6 | 27 | 100 | 0.0 | 0.0 | 0.0 | ||||||||||||
| cuptc321 | - | - | - | - | - | 101 | 55.4 | 3.0 | 4.0 | 37.6 | 114 | 67.6 | 1.7 | 1.7 | 29.0 | |||||||||||||
| cupty30b | - | - | - | - | - | 169 | 58.0 | 5.3 | 9.5 | 27.2 | 95 | 50.5 | 2.1 | 4.2 | 43.2 | |||||||||||||
L, left; B, bilateral; R, right; A, absent.
The mutant data above reflect the defects seen in clutches of embryos from a cross of tc321 and ty30b heterozygote parents. Therefore,the large percentage of left-sided expression of spaw and pitx2 is due to the approximate 75% of the population of embryos that are wild type or heterozygotes. Percentages of left-sided expression that are less than 75% may be attributed to errors in staging.