Summary of BrdU incorporation and cell density in limb buds of control,PC-NMycf/f and N-Myc null mice
| . | % BrdU positive . | . | . | Cells/equal area . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stage/position . | Wild type . | PC-NMycf/f . | N-Myc−/− . | Wild type . | PC-NMycf/f . | N-Myc−/− . | ||||
| E9.5 | 80±2.5 | 72±2.2** | 70±1.8** | 321±12 | 293±13** | 165±8* | ||||
| E10.5/Distal | 62±1.9 | 60±1.8*** | 60±1.5*** | 341±9 | 290±10** | 170±7* | ||||
| E10.5/Posterior | 63±1.3 | 59±2.1*** | NA | 344±12 | 265±13* | NA | ||||
| E10.5/Anterior | 65±1.6 | 59±1.8*** | NA | 351±13 | 308±9** | NA | ||||
| E11.5/Distal | 63±1.4 | 58±1.2*** | NA | 394±23 | 320±18** | NA | ||||
| E11.5/Posterior | 66±2.1 | 61±1.8*** | NA | 401±26 | 340±12** | NA | ||||
| E11.5/Anterior | 65±1.6 | 62±1.9*** | NA | 374±20 | 327±18** | NA | ||||
| . | % BrdU positive . | . | . | Cells/equal area . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stage/position . | Wild type . | PC-NMycf/f . | N-Myc−/− . | Wild type . | PC-NMycf/f . | N-Myc−/− . | ||||
| E9.5 | 80±2.5 | 72±2.2** | 70±1.8** | 321±12 | 293±13** | 165±8* | ||||
| E10.5/Distal | 62±1.9 | 60±1.8*** | 60±1.5*** | 341±9 | 290±10** | 170±7* | ||||
| E10.5/Posterior | 63±1.3 | 59±2.1*** | NA | 344±12 | 265±13* | NA | ||||
| E10.5/Anterior | 65±1.6 | 59±1.8*** | NA | 351±13 | 308±9** | NA | ||||
| E11.5/Distal | 63±1.4 | 58±1.2*** | NA | 394±23 | 320±18** | NA | ||||
| E11.5/Posterior | 66±2.1 | 61±1.8*** | NA | 401±26 | 340±12** | NA | ||||
| E11.5/Anterior | 65±1.6 | 62±1.9*** | NA | 374±20 | 327±18** | NA | ||||
Cell counts performed on limb buds of E9.5 embryos and N-Myc null embryos encompassed the entire limb bud. Otherwise, cell counts were performed on comparable distal, posterior and anterior regions as indicated. Cell counts were determined from stained nuclei within tissue sections derived from three embryos and standard deviations are shown. P values, *<0.01, ***>0.01. NA, not applicable.