Summary of the genetic experiments conducted and the resulting phenotypes
| Cre used (gene deleted) . | Domain of expression . | Single OFT at E18.5 . | Short OFT at E10.5 . | Abnormal arch arteries at E10.5/E18.5 . | AHF cell death . | NCC death . | Abnormal NCC localization/septation defect . |
|---|---|---|---|---|---|---|---|
| Nkx2.5Cre (Shh or Smo) | PE, AHF, specified myocardium, endocardium | + | + | +/+ | + | + | +/+ |
| Wnt1-Cre (Smo) | NCCs | + | − | +/+ | − | + | ?/+ |
| Mef2C-Cre (Smo) | AHF | + | − | −/+ | − | − | −/+ |
| TnT-Cre (Smo) | Specified myocardium | − | − | −/− | ND | ND | ND |
| Tie2-Cre (Smo) | Endocardium | − | − | −/− | ND | ND | ND |
| Cre used (gene deleted) . | Domain of expression . | Single OFT at E18.5 . | Short OFT at E10.5 . | Abnormal arch arteries at E10.5/E18.5 . | AHF cell death . | NCC death . | Abnormal NCC localization/septation defect . |
|---|---|---|---|---|---|---|---|
| Nkx2.5Cre (Shh or Smo) | PE, AHF, specified myocardium, endocardium | + | + | +/+ | + | + | +/+ |
| Wnt1-Cre (Smo) | NCCs | + | − | +/+ | − | + | ?/+ |
| Mef2C-Cre (Smo) | AHF | + | − | −/+ | − | − | −/+ |
| TnT-Cre (Smo) | Specified myocardium | − | − | −/− | ND | ND | ND |
| Tie2-Cre (Smo) | Endocardium | − | − | −/− | ND | ND | ND |
+, present; −, not present; ND, no data; ?, possibly; PE, pharyngeal endoderm; AHF, anterior heart field; NCC, neural crest cell.