Primer and probe sequences for quantitative PCR reaction
| Target . | Sense (5′-3′) . | Antisense (5′-3′) . | Probe . |
|---|---|---|---|
| P2X1 | GCTGATGGCTTGAGCCAG | TGGTGTCGCCTTGCAGGA | 6FAM-AGAGGGCATTCCCA |
| P2X2 | TCGACAAGGTGCGTACTCCA | GGCAAGGGTCACAGGCC | 6FAM-AGCATCCCTCAA |
| P2X3 | GCAGCGTACCGCACACT | ACCAGCACATCAAAGCGGA | 6FAM-CTGAAGGCTTTGGC |
| P2X4 | TGCAACCTGGATAGAGCC | GGCGCCGGAAGGAATATCT | 6FAM CTCCCTTTGCCTGCC |
| P2X5 | AGGCAGGAAAATTCAGCATCA | CGCCAGCCCAGAACCA | 6FAM-CCCACAGTCATCAAC |
| P2X6 | AACCAACTTCCTTGTGACACCA | GGAACAAGGATGCTCTGGG | 6FAM-TCAAGTCCAGGGCAGAT |
| P2X7 | GAGACGAACTGCGCATGTCA | TCCCCACATGTAACGACAAGG | 6FAM-AGAGGACCACTCTGCT |
| NSE | CCGCGATGGCAAATACG | TGCATCGGGTTGGGTCA | 6FAM-CTTGGATTTCAAGTCTCC |
| GAPDH | TGACAACTTTGGCATCGTGG | CACAGTCTTCTGAGTGGCAGTGAT | 6FAM-ATGACCACAGTCCATG |
| Target . | Sense (5′-3′) . | Antisense (5′-3′) . | Probe . |
|---|---|---|---|
| P2X1 | GCTGATGGCTTGAGCCAG | TGGTGTCGCCTTGCAGGA | 6FAM-AGAGGGCATTCCCA |
| P2X2 | TCGACAAGGTGCGTACTCCA | GGCAAGGGTCACAGGCC | 6FAM-AGCATCCCTCAA |
| P2X3 | GCAGCGTACCGCACACT | ACCAGCACATCAAAGCGGA | 6FAM-CTGAAGGCTTTGGC |
| P2X4 | TGCAACCTGGATAGAGCC | GGCGCCGGAAGGAATATCT | 6FAM CTCCCTTTGCCTGCC |
| P2X5 | AGGCAGGAAAATTCAGCATCA | CGCCAGCCCAGAACCA | 6FAM-CCCACAGTCATCAAC |
| P2X6 | AACCAACTTCCTTGTGACACCA | GGAACAAGGATGCTCTGGG | 6FAM-TCAAGTCCAGGGCAGAT |
| P2X7 | GAGACGAACTGCGCATGTCA | TCCCCACATGTAACGACAAGG | 6FAM-AGAGGACCACTCTGCT |
| NSE | CCGCGATGGCAAATACG | TGCATCGGGTTGGGTCA | 6FAM-CTTGGATTTCAAGTCTCC |
| GAPDH | TGACAACTTTGGCATCGTGG | CACAGTCTTCTGAGTGGCAGTGAT | 6FAM-ATGACCACAGTCCATG |