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Table 1.

Effects of mutations in the cell death machinery on tail-spike and pharyngeal cell death

Genotypea% surviving tail-spike cellsbAverage no. extra cells in anterior pharynx
A. egl-1 is partially required for tail-spike cell death   
Wild type 0±0c 0.5±0.2d 
ced-3(n717) 100±0 10.6±1.5 
ced-4(n1162) 100±0 11.1±1.1 
egl-1(n1084n3082) 30±6 11.1±1.5 
ced-13(tm536) 0±0 0.1±0.4 
ced-13(sv32) 0±0 0.2±0.4 
egl-1(n1084n3082); ced-13(tm536) 41±4 11.6±1.4 
B. egl-1 inhibits ced-9 to promote tail-spike cell death   
ced-3(n2427) 0±0 2.1±1.2 
ced-9(n2812); ced-3(n2427) 0±0 6.5±1.1 
ced-3(n2427); egl-1(n1084n3082) 100±0 11.2±1.8 
ced-9(n2812); ced-3(n2427); egl-1(n1084n3082) 0±0 5.2±1.8 
C. ced-9 lacks death-promoting activity in the tail-spike cell   
ced-3(n2436) 59±8 6.3±1.7 
ced-9(n2812); ced-3(n2436) 63±9 10.5±1.8 
D. A ced-9(gf) mutation weakly blocks tail-spike cell death   
ced-9(n1950gf) 3±3 10.3±1.3 
ced-9(n1950gf); ced-3(n2427) 50±9 11.5±1.1 
ced-9(n1950gf); ced-3(n2436) 93±5 11.0±1.8 
ced-5(n1812) 3±3 0.2±0.5 
ced-9(n1950gf); ced-5(n1812) 27±8 10.3±1.6 
ced-5(n1812); ced-3(n2427)e 17±7 5.1±1.8 
ced-9(n1950gf); ced-5(n1812) ced-3(n2427)e 73±8 11.1±1.5 
ced-9(n1950gf); egl-1(n1084n3082) 33±8 10.3±1.7 
Genotypea% surviving tail-spike cellsbAverage no. extra cells in anterior pharynx
A. egl-1 is partially required for tail-spike cell death   
Wild type 0±0c 0.5±0.2d 
ced-3(n717) 100±0 10.6±1.5 
ced-4(n1162) 100±0 11.1±1.1 
egl-1(n1084n3082) 30±6 11.1±1.5 
ced-13(tm536) 0±0 0.1±0.4 
ced-13(sv32) 0±0 0.2±0.4 
egl-1(n1084n3082); ced-13(tm536) 41±4 11.6±1.4 
B. egl-1 inhibits ced-9 to promote tail-spike cell death   
ced-3(n2427) 0±0 2.1±1.2 
ced-9(n2812); ced-3(n2427) 0±0 6.5±1.1 
ced-3(n2427); egl-1(n1084n3082) 100±0 11.2±1.8 
ced-9(n2812); ced-3(n2427); egl-1(n1084n3082) 0±0 5.2±1.8 
C. ced-9 lacks death-promoting activity in the tail-spike cell   
ced-3(n2436) 59±8 6.3±1.7 
ced-9(n2812); ced-3(n2436) 63±9 10.5±1.8 
D. A ced-9(gf) mutation weakly blocks tail-spike cell death   
ced-9(n1950gf) 3±3 10.3±1.3 
ced-9(n1950gf); ced-3(n2427) 50±9 11.5±1.1 
ced-9(n1950gf); ced-3(n2436) 93±5 11.0±1.8 
ced-5(n1812) 3±3 0.2±0.5 
ced-9(n1950gf); ced-5(n1812) 27±8 10.3±1.6 
ced-5(n1812); ced-3(n2427)e 17±7 5.1±1.8 
ced-9(n1950gf); ced-5(n1812) ced-3(n2427)e 73±8 11.1±1.5 
ced-9(n1950gf); egl-1(n1084n3082) 33±8 10.3±1.7 

aAll strains also contained the nsls25 or nsls23 C. briggsae ced-3 promoter::GFP integrated transgenes for scoring tail-spike cell survival. bBetween 20-40 L4 or L2 animals were scored for survival of pharyngeal or tail-spike cells, respectively, for each genotype. cStandard error of the mean. dMean±standard deviation. eContains unc-30(e191).

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